中文摘要
对黄花蒿种质资源调查，群落生态、生理生态、有效成分变异、遗传多样性等研究，探讨了青蒿素含量与生态因子的相关性；建立了青蒿素的高效液相色谱测定方法和成功地开发了8对黄花蒿微卫星（SSR）引物。明确优良黄花蒿种质。为优良黄花蒿种质的选育和保护利用提供科学依据。黄花蒿光合能力强，且对弱光的适应性也较强。其总生物量、叶生物量和青蒿素含量均随光强的增高而增大，强光有利于黄花蒿产量和有效成分含量增高。 青蒿素含量与土壤Ca的含量为正相关，与根N、茎N素含量以及植株地下部和地上部的N素含量比为负相关关系，与茎部和叶部的N素含量比有极显著负相关；植株叶片的N含量与土壤P含量有显著的正相关；影响青蒿素含量主要因子是其植株的养分含量，其次是土壤的养分含量和土壤和植株等综合因子。广西崇左市种群青蒿素平均含量最高，为0.932%，变异系数最小，变异系数为7.56%。陕西西安种群青蒿素平均含量最低，为0.016%，变异系数最大，变异系数为55.63%。子代青蒿素含量比亲本平均高12.65%，说明通过人工栽培可提高黄花蒿中的青蒿素含量。
英文摘要
The study focused on the main aspects of the resourcee, community ecology, photosysnthetic characteristics, composition variation and genetic diversity of Artemisia annua L. The relationships between the artemisinin content factors have been analysis and good Artemisia annua. germplasms have been choose. We develop an HPLC method to assess the content of Artemisinin and eight microsatellite markers from the genome of A. annua using the FIASCO protocol. It provided a scientific basis for the breeding and protection of good germplasms of Artemisia annua L.The photosynthesis properties of A. annua acclimated adequately to light conditions. At high light A. annua could utilize abundant light irradiance with high capacity of photosynthesis.With the increase of light intensity, the Total biomass, leaf bimass and Artemisinin content of A. annua enhanced.There was a notable positive correlation between the content of artemisinin and soil Ca，a notable negative correlation between the content of and artemisinin and root N，stem N and the ratio of N content in roots to that in the overground parts of the plant，a marked negative correlation between the ratio of stem N toleaf N ；And a notable positive correlation between the content of leaf N and soil P；The main factors affecting artemisinin accumulation were nutrient conte
结题摘要
对黄花蒿种质资源调查，群落生态、生理生态、有效成分变异、遗传多样性等研究，探讨了青蒿素含量与生态因子的相关性；建立了青蒿素的高效液相色谱测定方法和成功地开发了8对黄花蒿微卫星（SSR）引物。明确优良黄花蒿种质。为优良黄花蒿种质的选育和保护利用提供科学依据。黄花蒿光合能力强，且对弱光的适应性也较强。其总生物量、叶生物量和青蒿素含量均随光强的增高而增大，强光有利于黄花蒿产量和有效成分含量增高。 青蒿素含量与土壤Ca的含量为正相关，与根N、茎N素含量以及植株地下部和地上部的N素含量比为负相关关系，与茎部和叶部的N素含量比有极显著负相关；植株叶片的N含量与土壤P含量有显著的正相关；影响青蒿素含量主要因子是其植株的养分含量，其次是土壤的养分含量和土壤和植株等综合因子。广西崇左市种群青蒿素平均含量最高，为0.932%，变异系数最小，变异系数为7.56%。陕西西安种群青蒿素平均含量最低，为0.016%，变异系数最大，变异系数为55.63%。子代青蒿素含量比亲本平均高12.65%，说明通过人工栽培可提高黄花蒿中的青蒿素含量。