中文摘要
构建了我国首个谷子核心和微核心种质,分析了我国谷子农家品种和育成品种的遗传多样性;系统搜集了我国的谷子野生近缘种青狗尾草,初步形成了一个谷子野生种种质基础;原位杂交和分子标记分析鉴定出S.grisebachii具有不同于已知A和B基因组的新基因组C,S.queenslandica是A基因组的同源四倍体;在已知的三个基因组中,B和C之间有着较近的遗传关系,同A较远;开发谷子SSR标记1177对,并对其中166对进行了染色体定位;构建了谷子豫谷1号和青狗尾草青24的BAC文库各一个;利用EMS诱变,初步形成了一个含多种类型变异突变体库。本研究基本构建谷子功能基因分析的材料、标记和文库平台。
英文摘要
Foxtail millet, Setaria italica, is an ancient cereal which originated form China and contributed greatly to Chinese civilization development, and still an important crop in dry land of Northern China. A core collection and mini-core-collection of foxtail millet germplasm was constructed, which will for sure accelerate foxtail millet genetics and breeding. Over 3000 samples of foxtail millet wild ancestor, S. viridis, was firstly systematically collected from all over China, which establish the foundation of foxtail millet wild germplasm study. Gemones of the Setaria species was poorly studied, and we found a novel genome of C, carried by S.grisebachii, which is totally different from the known genome A and B. We also identified an A genome autotetroploid form an Australian species S.queenslandica, all those have for sure contributed Setaria genome evolution study. 1177 SSR markers were developed and among which 166 were anchored onto chromosome linkage group. Two BAC library, Yugu 1 and Q24, which represent foxtail millet and its wild ancestor, were constructed in the program. Mutant lines are key issues for functional genomic study. We have developed many mutant lines of foxtail millet by EMS treatment and most of them were ready to be used in the following functional genomic research.
结题摘要
构建了我国首个谷子核心和微核心种质,分析了我国谷子农家品种和育成品种的遗传多样性;系统搜集了我国的谷子野生近缘种青狗尾草,初步形成了一个谷子野生种种质基础;原位杂交和分子标记分析鉴定出S.grisebachii具有不同于已知A和B基因组的新基因组C,S.queenslandica是A基因组的同源四倍体;在已知的三个基因组中,B和C之间有着较近的遗传关系,同A较远;开发谷子SSR标记1177对,并对其中166对进行了染色体定位;构建了谷子豫谷1号和青狗尾草青24的BAC文库各一个;利用EMS诱变,初步形成了一个含多种类型变异突变体库。本研究基本构建谷子功能基因分析的材料、标记和文库平台。
