由大肠杆菌引起的仔猪腹泻仍是一个没有完全解决的问题，给养猪业带来的损失不可低估；新近发现的携带耶尔森氏菌强毒力岛（HPI）的大肠杆菌使得仔猪腹泻的病原更具复杂性。为进一步做好仔猪腹泻的有效防控，该项目将从以下3个方面对HPI+大肠杆菌进行深入研究：（1）利用本实验室建立的检测方法，确定HPI在猪源大肠杆菌基因组中的定位，深入分析HPI+大肠杆菌的分子流行病学，确定其在致仔猪腹泻病原菌中的地位；（2）以本实验室已分离鉴定的10株HPI+大肠杆菌为材料，确定其在实验动物和仔猪中的致病作用；（3）选择2株HPI+大肠杆菌为对象，通过对HPI的关键结构基因（如ipr1、irp2、fyuA、ybtA）的敲除和敲回，确定HPI毒力岛对大肠杆菌毒力的贡献。该项目将对推动我国集约化养猪业的健康发展具有重要的指导价值。

Porcine diarrhea caused by Escherichia coli is a common disease and receiving increased attention due to the high economic losing. Recently, a novel Escherichia coli contained High Pathogenicity Island (HPI) was identified in pigs with diarrhea, which make the pathogenic agents more perplexing. Some of this agent is poorly studied, and/or its significance to swine health is unknown. To further prevent and control the diarrhea caused by HPI-positive E.coli, this research will be developed by following work: (1) Investigate into the prevalence of HPI factors in E.coli isolates, make known the HPI element loci on the E.coli genome, detail the role of HPI-positive E.coli in the porcine diarrhea. (2) Determine the pathogenicity of HPI in laboratory animals and piglets by using 10 HPI-positive isolates. (3) Demonstrate the contribution of HPI to the pathogen virulence by knocking out and/or knocking in the key genes, such as irp1, irp2, fyuA and/or ybtA.

由大肠杆菌引起的仔猪腹泻仍是一个没有完全解决的问题，给养猪业带来的损失不可低估；新近发现的携带耶尔森氏菌强毒力岛（HPI）的大肠杆菌使得仔猪腹泻的病原更具复杂性。为进一步做好仔猪腹泻的有效防控，该项目将从以下3个方面对HPI+大肠杆菌进行深入研究：（1）利用本实验室建立的检测方法，确定HPI在猪源大肠杆菌基因组中的定位，深入分析HPI+大肠杆菌的分子流行病学，确定其在致仔猪腹泻病原菌中的地位；（2）以本实验室已分离鉴定的10株HPI+大肠杆菌为材料，确定其在实验动物和仔猪中的致病作用；（3）选择2株HPI+大肠杆菌为对象，通过对HPI的关键结构基因（如ipr1、irp2、fyuA、ybtA）的敲除和敲回，确定HPI毒力岛对大肠杆菌毒力的贡献。该项目将对推动我国集约化养猪业的健康发展具有重要的指导价值。