该基金项目的工作包含以下二部分：.一、特殊序列DNA传感器的制备：（1）即利用碳纳米管、金纳米层层组装的方式（静电作用，共价键）构筑特殊序列的DNA电化学传感器。用循环伏安，电化学阻抗谱来表征组装过程，以阿霉素作为杂交指示剂。差示脉冲伏安技术用于DNA 的杂化过程中信号的变化。（2）构筑了基于金纳米粒子修饰电极的电化学阻抗传感器。利用DNA杂化前后电子传递电阻的差异进行检测。（3）利用导电聚合物为基底，组装碳纳米管、金纳米粒子传感界面构建DNA生物传感器；差示脉冲技术，计时库仑技术用于检测DNA的杂化。制备的传感器灵敏度高，选择性好及稳定性好。.二、以碳纳米管、血红蛋白/肌红蛋白、表面活性剂为材料，采用层层组装技术构建生物小分子（多巴胺、尿酸）及西咪替丁、过氧化氢，三氯乙酸的生物传感器；用扫描电镜、电化学阻抗谱、紫外可见、红外光谱研究了膜组装过程，研究了肌红蛋白的直接电化学，同时探讨了该修饰电极对过氧化氢，氯乙酸的电催化作用；研究了西咪替丁与血红蛋白的相互作用。

This work contains two aspects: one is the praparation of electrochemical DNA biosensor for special sequence.(1) Fabrication of a sensitive electrochemical DNA biosensor based on multifilms of MWCNT and gold nanoparticles by layer-by-layer (LBL) assembly techniques( Covalent or electrostatic mode).The stepwise LBL assembly process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).The hybridization events were monitored by differential pulse voltammetry (DPV) measurement of the intercalated adriamycin.(2) Fabrication of a sensitive impedance biosensor of DNA hybridization based on gold nanoparticles modified gold electrode.EIS was used to investigate probe DNA immobilization and hybridization.(3)Fabrication of a electrochemical DNA biosensor based on conductive polymer, CNTs and gold nanoparticle. Adriamycin and [Ru(NH3)6]3+ are used for hybridization indicator. DPV and CC techniques are used for the detection of DNA hybridization.these biosensor shows good sensitivity, selectivity and stability.. Others is the fabrication of small molecule biosensor with multilayer films modified electrode composed of myoglobin, chitosan, and CNT by layer-by-layer assembled technique.The assembled process was characterized with CV,SEM,UV and EIS techniques.We investigate their interaction between Mb and cimetidine. We have studied the electrochemical catalysis for hydrogen peroxide and trichloroacetic acid.

该基金项目的工作包含以下二部分：一、特殊序列DNA传感器的制备：（1）即利用碳纳米管、金纳米层层组装的方式（静电作用，共价键）构筑特殊序列的DNA电化学传感器。用循环伏安，电化学阻抗谱来表征组装过程，以阿霉素作为杂交指示剂。差示脉冲伏安技术用于DNA 的杂化过程中信号的变化。（2）构筑了基于金纳米粒子修饰电极的电化学阻抗传感器。利用DNA杂化前后电子传递电阻的差异进行检测。（3）利用导电聚合物为基底，组装碳纳米管、金纳米粒子传感界面构建DNA生物传感器；差示脉冲技术，计时库仑技术用于检测DNA的杂化。制备的传感器灵敏度高，选择性好及稳定性好。二、以碳纳米管、血红蛋白/肌红蛋白、表面活性剂为材料，采用层层组装技术构建生物小分子（多巴胺、尿酸）及西咪替丁、过氧化氢，三氯乙酸的生物传感器；用扫描电镜、电化学阻抗谱、紫外可见、红外光谱研究了膜组装过程，研究了肌红蛋白的直接电化学，同时探讨了该修饰电极对过氧化氢，氯乙酸的电催化作用；研究了西咪替丁与血红蛋白的相互作用。