中文摘要
本课题通过优化标记体系，确立了适用于乳杆菌的ERIC-PCR和AFLP两种分子标记的具体实验方法，完成对来自不同地区自然发酵乳中的乳杆菌进行遗传多样性分析。1 对115株乳杆菌西藏分离株进行多样性研究。ERIC-PCR以同源率0.80为分界点，被分为14个群。AFLP以同源率0.85为分界点，菌被分成4个AFLP群。2 对81株乳杆菌新疆分离株进行多样性研究。ERIC-PCR以同源率0.76为分界点，被分为4个群。AFLP以同源率0.80为分界点，被分为6个群。3 对69株乳杆菌云南分离株进行多样性研究。ERIC-PCR以同源率0.80为分界点，被分为4个群。AFLP以同源率0.89为分界点，被分成5个AFLP群。4 对西藏、新疆和云南三个地区的105株瑞士乳杆菌分离株进行遗传多样性分析。以同源率0.80为分界点，分别形成7个ERIC-PCR群和5个AFLP群。并且不同地区分离株形成不同的类群。通过分析，两种分子标记技术都具有高的遗传多态性，可用于乳杆菌基因组水平的多态性分析。
英文摘要
In this study, ERIC-PCR and AFLP molecular markers which were suitable for Lactobacillus were established. The genetic diversity of 316 Lactobacillus isolated from different regions traditional fermented dairy products was completed using by the two methods.1. The genetic diversity of 115 Lactobacillus isolated from Tibet was dedected. The tested strains were clustered into 14 groups by ERIC-PCR at similarity level of 0.80, and were clustered into 4 groups by AFLP at similarity level of 0.85.2. The genetic diversity of 81 Lactobacillus isolated from Xinjiang was dedected. The tested strains were clustered into 4 groups by ERIC-PCR at similarity level of 0.76, and were clustered into 6 groups by AFLP at similarity level of 0.80.3. The genetic diversity of 69 Lactobacillus isolated from Yunnan was dedected. The tested strains were clustered into 4 groups by ERIC-PCR at similarity level of 0.80, and were clustered into 5 groups by AFLP at similarity level of 0.89.4. The genetic diversity of 105 Lactobacillus helveticus isolated from Tibet，Xingjiang, and Yunnan were analyzed. The tested lactobacillus helveticus were clustered into 7 ERIC-PCR groups and 5 AFLP groups at the similarity level of 0.80, respectively. And the strains isolated from different geographic origin could be divided into different group by tw
结题摘要
本课题通过优化标记体系，确立了适用于乳杆菌的ERIC-PCR和AFLP两种分子标记的具体实验方法，完成对来自不同地区自然发酵乳中的乳杆菌进行遗传多样性分析。1 对115株乳杆菌西藏分离株进行多样性研究。ERIC-PCR以同源率0.80为分界点，被分为14个群。AFLP以同源率0.85为分界点，菌被分成4个AFLP群。2 对81株乳杆菌新疆分离株进行多样性研究。ERIC-PCR以同源率0.76为分界点，被分为4个群。AFLP以同源率0.80为分界点，被分为6个群。3 对69株乳杆菌云南分离株进行多样性研究。ERIC-PCR以同源率0.80为分界点，被分为4个群。AFLP以同源率0.89为分界点，被分成5个AFLP群。4 对西藏、新疆和云南三个地区的105株瑞士乳杆菌分离株进行遗传多样性分析。以同源率0.80为分界点，分别形成7个ERIC-PCR群和5个AFLP群。并且不同地区分离株形成不同的类群。通过分析，两种分子标记技术都具有高的遗传多态性，可用于乳杆菌基因组水平的多态性分析。