中文摘要
氧化还原蛋白质的直接电化学和高灵敏、宽线性范围电化学生物传感器的研究已经成为电分析化学家研究的热点领域。本项目构置了6种基于Sol-Gel的修饰陶瓷碳电极，17种基于离子液体的离子液体碳糊电极、离子液体/生物材料和离子液体/纳米材料修饰电极，1种基于电磁控制的修饰电极，并开展了小分子物质、有机药物和氧化还原蛋白质（酶）等的电化学行为及电催化研究；建立了过氧化氢、抗坏血酸、肼、半胱氨酸等的安培检测新方法，使相关测定灵敏度提高1~2个数量级、线性范围可达3~4个数量级，稳定性与重复性更好。项目中取得的研究成果，为生命科学中氧化还原蛋白质的电子传递作用机理研究提供了新的手段，对丰富化学修饰电极技术与理论、拓展电化学分析技术在生物领域中的应用范围具有重要意义。在《Biosensors and Bioelectronics》和《Electrochemistry Communications》等杂志上共发表论文37 篇。
英文摘要
The study of direct electrochemistry and electrochemical biosensors with high sensitivity and wider linear range has become one of the research subjects for electroanalysts. In this project, six kinds of chemically modified electrodes based on sol-gel derived carbon ceramic electrodes, seventeen kinds of modified electrodes based on ionic liquid carbon paste electrodes, ionic liquid/biomaterials and ionic liquid/nanomaterials, and one kind of modified electrodes based on electromagnetic control were fabricated. The electrochemical behaviors and electrocatalytic properties of small molecules, organic drugs and redox proteins (enzymes) were further studied, and on this basis, new amperometric methods for the determination of H2O2, ascorbic acid, N2H4 and cysteine were established. Moreover, the sensitivity can be risen one or two orders of magnitude, the linear range can be covered three or four orders of magnitude and the methods showed good stability and reproducibility. The all achievements obtained from this item meliorated and developed the theory and the techniques of novel modified electrodes, broadened the application range and enlarged the research fields of biosensing interfaces, at the same time provided the new means of biologic active substances and the electron transfer mechanism of redox proteins in life science. During this item, 37 papers were published on the magazines such as《Biosensors and Bioelectronics》and《Electrochemistry Communications》.
结题摘要
氧化还原蛋白质的直接电化学和高灵敏、宽线性范围电化学生物传感器的研究已经成为电分析化学家研究的热点领域。本项目构置了6种基于Sol-Gel的修饰陶瓷碳电极，17种基于离子液体的离子液体碳糊电极、离子液体/生物材料和离子液体/纳米材料修饰电极，1种基于电磁控制的修饰电极，并开展了小分子物质、有机药物和氧化还原蛋白质（酶）等的电化学行为及电催化研究；建立了过氧化氢、抗坏血酸、肼、半胱氨酸等的安培检测新方法，使相关测定灵敏度提高1~2个数量级、线性范围可达3~4个数量级，稳定性与重复性更好。项目中取得的研究成果，为生命科学中氧化还原蛋白质的电子传递作用机理研究提供了新的手段，对丰富化学修饰电极技术与理论、拓展电化学分析技术在生物领域中的应用范围具有重要意义。在《Biosensors and Bioelectronics》和《Electrochemistry Communications》等杂志上共发表论文37 篇。