基于本课题组在前期研究工作中已分离获得的异养菌株Bacillus SP. LY所表现出的异养硝化及脱氮性能，首次提出异养脱氮的概念。通过不在同培养条件下异养硝化和好氧反硝化功能的研究，确认Bacillus sp. LY菌株异养脱氮的代谢机制。采用超声波酶破碎技术，对Bacillus sp. LY菌株的胞内酶进行粗提，测定了异养脱氮过程中关键酶（羟胺氧化酶HAO，亚硝酸盐还原酶NiR和硝酸盐还原酶NR）的酶活性及酶比活力，从酶学上进一步描述Bacillus sp. LY异养脱氮机制。在研究Bacillus sp. LY 菌株的过程中，我们还分离出两株新型的异养脱氮菌株，分别属于Acinetobacter calcoaceticus 和Providencia rettger，这进一步证明了异养脱氮的存在。而且，在研究过程中，我们发现了不同于传统方式的新型脱氮途径，这为进一步深入了解异养脱氮，开发新型高效生物脱氮工艺建立了研究基础。

Based on the previous research of heterotrophic nitrification and nitrogen removal by Bacillus sp.LY, a novel concept of heterotrophic nitrogen removal has been proposed for the first time. The research focused on the capability of heterotrophic nitrification and aerobic denitrification under different culture conditions by strain LY, and thus, the metabolic mechanism of heterotrophic nitrogen removal was analyzed. Based on the ultrasonication technology, crude extracts of strain LY were obtained and the enzyme activities of hydroxylamine oxidase (HAO), nitrate reductase (NR) and nitrite reductase (NiR) were detected. Thus, the metabolic mechanism of heterotrophic nitrogen removal was further confirmed. Another two bacteria belonging to Acinetobacter calcoaceticus and Providencia rettger were isolated during the research on Bacillus sp. LY. Therefore, the existence of heterotrophic nitrogen removal was further confirmed. Moreover, a novel nitrogen removal pathway, which was quite different from the conventional nitrogen removal pathway, was found. All these results may contribute to the establishment of a novel biology process for nitrogen removal with high efficiency.

基于本课题组在前期研究工作中已分离获得的异养菌株Bacillus SP. LY所表现出的异养硝化及脱氮性能，首次提出异养脱氮的概念。通过不在同培养条件下异养硝化和好氧反硝化功能的研究，确认Bacillus sp. LY菌株异养脱氮的代谢机制。采用超声波酶破碎技术，对Bacillus sp. LY菌株的胞内酶进行粗提，测定了异养脱氮过程中关键酶（羟胺氧化酶HAO，亚硝酸盐还原酶NiR和硝酸盐还原酶NR）的酶活性及酶比活力，从酶学上进一步描述Bacillus sp. LY异养脱氮机制。在研究Bacillus sp. LY 菌株的过程中，我们还分离出两株新型的异养脱氮菌株，分别属于Acinetobacter calcoaceticus 和Providencia rettger，这进一步证明了异养脱氮的存在。而且，在研究过程中，我们发现了不同于传统方式的新型脱氮途径，这为进一步深入了解异养脱氮，开发新型高效生物脱氮工艺建立了研究基础。