少根紫萍淀粉积累快含量高，已成为关注热点，但其淀粉积累机制尚不清楚。我们在筛选获得的少根紫萍的株系上发现其分别在寡营养和烯效唑处理下10天内，淀粉含量从3%分别达到45%和48%，并采用组学等多种技术初步解析了淀粉代谢通路的变化。进而又发现寡营+烯效唑协同处理能够更大幅度地提高少根紫萍的淀粉积累能力, 10天内其淀粉含量达到72%以上，淀粉产量比对照提高了50倍以上，因此是研究淀粉快速高效积累的理想材料。本项目针对寡营养+烯效唑协同处理下少根紫萍淀粉快速大量积累的现象，从碳固定、生物量、淀粉含量和产量、淀粉代谢与碳固定的关键酶系、淀粉代谢途径及碳固定通路的转录组以及蛋白质组等方面系统研究，结合已完成的全基因组数据与前期的寡营养和烯效唑处理的结果进行比较分析，深度揭示少根紫萍淀粉快速大量积累的机制，为开发高淀粉浮萍生物材料提供理论依据，也为其它植物实现快速大量淀粉积累提供有益参考。

Landoltia punctata is generally recognized as a potential aquatic plant for high content and rapid accumulation of starch. However, there is little research about molecular mechanism of its starch metablism. In previous works, we have obtained Landoltia punctata strains with high starch contents by systematic screening, and their characteristics of starch accumulation under different treatment and regulation conditions were studied. When the strains were treated with nutrient starvation or uniconazole, the starch contents could reach 45 % and 48 % from 3%, respectively, in10 days. The changes of starch metabolism pathway under these conditions have been elucidated respectively using a variety of techniques included omics. Recently, we found the starch accumulation could be improved much more (reached 72% in 10 days) when the ducweed strains were cooperatively treated with nutrient starvation and uniconazole simultaneously. So in this application, we intend to study the strains cooperatively treated with uniconazole + nutrient starvation at different levels (including carbon storage biomass and starch accumulation, analysis of enzymes involved in starch biosynthesis and degradation, transcriptome and the proteome of starch metabolic pathway and carbon fixation pathway), then the results obtained through this project will be comparatively analyzed with those previously obtained by nutrient starvation and uniconazole treatment. These works would be beneficial for us to reveal the common mechanism of their starch metabolism and to discover some common Transcription factors. The results will be very important for developing Landoltia punctata as a high-starch bio-material for wide usage such as bio-ethanol production, and will also provide us some useful clues for manipulating other plants to produce starch higher and more quickly.