海洋酸化使多种海洋生物乃至生态系统面临巨大威胁。海洋微藻对海洋酸化的响应与调节在全球环境变化中发挥着重要作用。微拟球藻是一种具有高营养价值的饵料微藻和最有潜力的工业产油模式微藻，具有重要研究价值。面对不可逆转的海洋酸化现象，微拟球藻会有什么样的生理响应和分子调节机制目前还缺少系统的研究。本项目将海洋酸化为研究背景，拟采用生理生化检测和分子生物学手段与组学数据相结合的方法，研究微拟球藻对短期酸化环境的应急生理生化响应及对长期酸化环境的适应及进化机制。研究不同酸化时间和尺度下，微拟球藻对海洋酸化的响应过程和规律，借助高通量测序技术，通过比较转录组数据，分析微拟球藻在酸化条件下主要代谢途径的变化，阐释其分子调控机制。同时，通过动物喂养实验，分析海洋酸化过程中微拟球藻的变化可能对食物链的影响。 本项目的实施将为全面解析微拟球藻应对全球气候变化的生物学机制研究提供理论依据。

Ocean acidification made a variety of marine organisms and ecosystems facing a great threat. Response and regulation of marine microalgae to ocean acidification play an important role in global environmental change. Nannochloropsis ,a kind of high nutritional value bait microalgae and most potential mode of industrial oil microalgae, have important research value.In the face of inevitable phenomenon of ocean acidification, what physiological response and molecular regulation mechanism to ocean acidification in Nannochloropsis are still lack of systematic study. Under the background of ocean acidification, physiological and biochemical detection and molecular biological method combining the omics data are proposed in this project. We will study immediate physiological response to long-term ocean acidification and the adaptive response mechanism to long-term ocean acidification in Nannochloropsis. The response process and rule to ocean acidification in Nannochloropsis under different acidification time and scales will be stuied. With the aid of high-throughput sequencing technologies, by comparing the transcriptome data and analying the of the change of main metabolic pathways in Nannochloropsis under the condition of ocean acidification, we will try to interpret its molecular regulatory mechanism. At the same time, through animal feeding experiment,we will analyze the possible effect on the food chain causing from the changes in Nannochloropsis found in the process of ocean acidification . The implementation of this project will provide theory basis for comprehensively analyzing the biological mechanism in dealing with global climate change in Nannochloropsis.