第四纪以来异质性生境和气候变化对青藏高原地区和西部荒漠地区分布植物物种的遗传多样性和物种演化是否具有相同的作用是值得深入研究的重要课题。本项目拟选择锦鸡儿属近缘种鬼箭锦鸡儿和荒漠锦鸡儿进行研究，比较其种内种间遗传多样性分布规律及应对环境和气候变化的适应机制。广布于青藏高原的鬼箭锦鸡儿和西北荒漠地区主要建群种之一荒漠锦鸡儿在该属中起源较早，且受青藏高原隆升影响深广。通过简化基因组测序对中性与适应性基因分别构建系统发育树，并进行群体遗传结构分析，阐明两个近缘物种异域分布在不同生境的种群，在基因流水平、选择作用强度等方面的遗传变异规律，全面了解种群现状并追溯现代分布种群的进化历程。结合环境因子比较高原和旱区分布植物在第四纪和近期的环境变化中的气候响应趋势和遗传多样性的变化式样，揭示近缘物种适应不同非生物环境的进化机制，同时也为锦鸡儿属物种的种质资源保护和利用提供理论支持。

It remains elusive whether the plants occurring in the Qinghai-Tibetan Plateau and western desert regoin shared the common responses to the climatic changes and environmental heterogeneity since Quaternary. Then microclimatic caused by landscape changes and human activities could also promoted intra and interspecific divergence. In the present project, we selected Caragana jubata. and C. roborovskyi to compare the genetic diversity and climate adaptation inter- and intraspecies，which based on GBS analysis. As closely related Eurychoric Caragana species-pairs, they respectively adapted in the Qinhai-Tibetan plateau alpine steppe and become constructive species in the desert region of Northwest China. It seemed that early origin but differentiated gradually influenced by the microenvironments due to the qinghai-tibet plateau uplift. We will apply genotyping by sequencing (GBS) to detect genetic diversity, gene flow, and selection of individuals between and within populations, firstly compare phylogenetic tree and analysis phylogeographic structures, which build on neutral and non-neutral SNP pairs; secondly, clarify all historical changes and evolutionary process that resulted in the current distributions of two species. It will be a good case of studying patterns of genomic divergence response to climatic changes, and may shed light on the underlying evolutionary processes: Since Quaternary till now, the alpine and desert Caragana species share the same pattern of genetic diversity, or not? Such detailed comparisons will effectively reveal the adaptation mechimanism of these two plants, and also for further conservation and unitilization of these Caragana species resources.