中文摘要
被誉为“原始森林”的巩乃斯林场在天山西南部,位于新疆和静县境内。2003年被国家林业局批准为国家级森林公园。经考察发现,该森林内死亡的树或朽木做为栖息地的附生植物的地衣种类多,并地衣多样性往往高于生活的树木上生活的地衣种类。也是中国濒危地衣研究中的一个重要地区。本研究基于新疆的地衣研究现状,采用形态解剖、化学、生态类型及地理成分分析等传统分类方法与现代分子系统学研究方法相结合,对该地区地衣进行分类研究,以搞清楚该地区地衣的属种数目。在室内,首先对地衣体、子囊盘及分生孢子器的内部结构等进行观察;采用显色反应法、薄层层析法等生物化学方法,对地衣所特有的地衣酸种类进行鉴定;用分子生物学方法进行DNA序列分析,以确定新种和难鉴定种的系统学地位;最后对地衣生态特征和地衣地理分布成分分析。研究结果为整个新疆地衣区系研究、中国地衣志的编写提供科学性资料,同时为国家地衣资源的保护与利用提供基本数据。
英文摘要
Gongnaisi Forest Farm is known as a " Old-growth forest",and is Located in nourthwest of Hejing County,Xinjiang, China. Approved as a national Forest Park by the State Forestry Administration in 2003. By investigation, found dead and dying trees are a favorite habitat of many epiphytic lichens in this area and lichen diversity is often higher than on living, vital trees.Hence, the presence of old dying trees and snags has repeatedly been emphasized as an important goal for the conservation of forest ecosystems. Over the last ten years, the forests of the west Tianshan Mt.s are encountering some new problems: environmental stress (air pollution) and development of tourism and human activity. The composition of this flora has changed drastically during recent years. Recently several protected areas were established , but the problem of nature protection in the west part of Tianshan Mts remains urgent. Therefore, any information on the regional biodiversity, especially on the distribution of rare and endangered species in China, is important. This research is based on the present research of Xinjiang lichens, with reference to methods and contents of China's other provinces and international lichen researchers, this research aims at the Classification of lichens in the area, clear the area’s lichen species. Morphological and anatomical observations were made using stereoscopic and compound microscopes. Hand-cut sections mounted in water were made for anatomical observations and measurements of ascomatal structures.The granules and crystals were observed with the aid of polarizing filters and its solubility was valuated in K and N. Secondary metabolites were identified by using spot tests reactions: K(potassium hydroxide solution), C(sodium hypochlorite solution), KC and Pd (paraphenilenediamine), UV fluorescence, thin layer chromatography(TLC). Using molecular biology methods to formulate the new species systematic position.Also study the lichen growth forms,lichen ecological characteristics and analysis the lichen geographic component.The results of the research will be provides scientific information to the writing the Flora of China lichens and continuing studies of the lichen flora of Xinjiang.
