花器官的不对称性决定了花型结构，包括背腹不对称（Dorsal-Ventral asymmetry，DV）、花瓣内部不对称（Internal asymmetry，IN）以及左右不对称（Left-Right asymmetry，LR）三种发育途径，其中DV和IN途径已得到广泛研究，但参与LR途径的基因尚未见报道。本课题组前期以绿豆为模式植物，首次诱变筛选到了花器官LR途径受到改变的突变体sif（左右两侧的侧部与腹部花瓣发生左右倒转），并成功克隆到了SIF基因，进一步的研究表明SIF属于植物所特有的SPR1类微管结合蛋白。本研究拟在前期工作基础上，通过二次诱变筛选、生物信息学分析、酵母双杂交等技术手段，鉴定SIF的下游因子和互作蛋白质，以阐明SIF参与LR发育的分子途径及遗传调控网络，为进一步揭示植物花器官LR发育的分子机理提供理论依据。

Floral organ asymmetry determines the final pattern structure of flower, including Dorsal-Ventral asymmetry(DV)、Internal asymmetry(IN) and Left-Right asymmetry(LR) development processes. There are many studies during DV and IN pathways on flower development while the key factors participate in LR pathway has not cloned. We used mungbean as model plant and carried out a large-scale γ-ray mutagenesis experiment. Fortunately, we obtained a mutant named sif which stunt LR pathway（both of the lateral and ventral petals reverse from the left side to the right side）. Use map-based cloning method, we cloned SIF and sequence analysis showed that it belongs to SPR1 family. The objective of the present work was to establish the genetic network, identify the downstream targets and interacted proteins of SIF by genetics, molecular biology and bioinformatics. Our study will reveal the genetic network and molecular mechanism of SIF controlling the LR floral development in mungbean and provides an entirely new picture to understand flower Left-Right asymmetry morphogenesis.