物种的分布范围和遗传格局受地质历史事件和环境变迁的深刻影响。关于冰川时期中国东海陆桥在物种迁徙中发挥的作用仍存在争议。东亚野生萝卜(Raphanus sativus var. raphanistroides Makino)横跨中国东海分布，生活周期较短，对环境变化的敏感性强，并且在日本表现出独特的进化模式，是研究东亚植物区系物种形成与演变历史的理想材料。本项目拟采用ddRADseq和叶绿体全基因组测序技术，检测野生萝卜的遗传多样性及谱系分化，确定种群历史动态，揭示冰期避难所及冰期后的迁移路线；并结合生态位模拟和景观遗传的方法对物种各时期（末次盛冰期、当代和将来）的潜在分布区进行模拟重建，分析物种适应性遗传变异的驱动因子，揭示地质历史事件与生态因子对其物种形成与演化的塑造作用，阐明中国东海陆桥在野生萝卜冰期迁移中发挥的作用，为东亚植物区系的物种发生和演化机制提供新的证据。

The distribution range and genetic pattern of species are deeply affected by geological history events and environmental changes. The role of the East China Sea land bridge in species migration in glacial period is still controversial. East Asian wild radish (Raphanus sativus var. raphanistroides Makino) distributed across the East China Sea, represents a short life cycle and high sensitivity to environmental changes, and shows a unique evolutionary pattern in Japan, thus it is an ideal material to study the East Asian flora species formation and evolutionary history. In this project, ddRADseq and chloroplast genome sequencing techniques will be used to detect the genetic diversity and lineage divergence of wild radish, to identify the population dynamics history, and to reveal the migration route and refugia during the glacial period. In addition, ecological niche model and landscape genetic methods will be combined to simulate and analyze the potential distribution of wild radish in each period (LGM, current and future), to address the driving factors for adaptive genetic variation of species, to reveal the effects of geological history events and ecological factors on the speciation and evolution of wild radish, and to clarify the role of the East China Sea land bridge in the migration of wild radish in glacial period. This study will provide new insights into underlying mechanism of speciation and adaptive evolution of plants in East Asia.