维吾尔药睡莲花具有降热养肝、消炎止咳之功效，用于干热性肝虚等病症的治疗。前期实验显示睡莲花有效部位/成分对小鼠化学性和免疫性肝损伤均有较为显著的防治作用；通过体外试验还发现其有效部位/成分对肝星状细胞的增殖具有显著的抑制作用。然而，该药材抗肝纤维化作用的研究却鲜有文献报道。因此，本项目拟通过各种色谱技术分离纯化睡莲花中的有效部位/成分，并进一步采用四氯化碳诱导的大鼠肝纤维化模型，通过生化指标检测及病理组织学观察，在整体水平上考察有效部位/成分对大鼠肝纤维化的作用及其作用机制。体外选用HSC-T6细胞株，通过观察效有效部位/成分对HSC-T6增殖、凋亡和胶原分泌的作用，以及对NF-κB、PDGF和TGF-β1/Smad信号传导通路的影响，揭示睡莲花有效部位/成分的作用靶点；并从细胞和分子水平上阐明其抗肝纤维化的分子机制。结果可为维吾尔药睡莲花的深入研究及其新药开发提供基础数据。

Nymphaea candida possessed the efficacy of clearing away heat, tranquilization, nourishing the liver, and relieving inflammation, and was used to treat various disease in Uighur medicine. Previous studies have shown that some extracts and constituents from N. Candida have significantly preventive and therapeutic effect against chemical and immunological liver injury. Though biological activities screening experiment in vitro, it was found that these extracts and constituents not only could inhibit proliferation of hepatitis stella cell (HSC), but also inhibit the expression of Smad 2/3 protein and promote the expression of Smad 7 protein. However, anti-hepatic fibrosis effective of this plant was rarely reported. Therefore, This project aims to investigate anti-hepatic fibrosis effect of N. candida against CCl4 induced hepatic fibrosis in rat and its mechanism on the basement of isolation and purification of active fraction/compounds. Moreover, the anti-hepatic fibrosis effective target of N. candida and its molecular mechanism were further studied by apoptosis of HSC-T6, secretion of collagen as well as NF-κB、PDGF and TGF-β1/Smad signal transduction pathways in HSC-T6. The results will provide a theoretical basis for the further expansion of N. candida in the medical field as well as development of new drugs on prevention and treatment of liver fibrosis.