中文摘要
提高大豆单产对我国大豆生产安全具有重要的战略意义。营养生长时期长,开花晚,可以提高作物产量,但在生产上无法应用;而适当延迟开花时间则具有潜在高产效应。Flowering Locus T(FT)是一个最关键的成花素基因。FT基因的突变导致晚花、营养体健壮; FT 突变基因的杂合体在水稻和番茄中具有显著的高产表型。这表明,精细调节FT基因的表达量有望成为提高作物产量的有效途径。我们前期工作获得大豆FT同源基因GmFTL(Glycine max FTL-Like)的RNAi转基因大豆(GmFTL-RNAi大豆),它们在不同条件下都具有显著的高产表型。本项目将根据文献报道的线索,通过形态解剖学、生理学、转录组学、代谢组学和分子生物学等方法,综合解析GmFTL-RNAi大豆的高产机制,试图建立GmFTL对光合作用的调控途径;并且通过田间实验,探索GmFTL-RNAi大豆的高产栽培方案。
英文摘要
It has strategic significance for China soybean production to improve soybean yield. Long vegetative phase results in high yield, but it is unsuitable in agriculture due to much later flowering. However, extending vegetative phase to a suitable extend may be a potential way to improve yield without cost of much late flowering in crop. Flowering Locus T (FT), a florigen gene, is a key integrator for flowering multiple-pathways. FT mutation results in later flowering and robust and healthy stature. Heterozygous of ft mutant exhibits significantly high yield. It indicates that fune-tuning FT-expression may be an efficient approach to improve crop yield. Our previous work showed that silencing of soybean FT homologs (GmFTL, Glycine max FTL-Like) resulted in distinctly high yield in different conditions. This proposal aims to uncover high-yield mechanism of GmFTL-RNAi soybean through a series of approaches in morpho-anatomy, physiology, transcriptome, metabolome, and molecular biology, and try to establish a planting proposal through semiplant test.
