绿原酸在体外具有抗癌、抗炎、抗菌、抗病毒等广泛的药理学作用。烟草是绿原酸的含量较高的植物之一，是大批量提取绿原酸的理想植物。目前已知，HQT基因可调控植物绿原酸合成，并且推测HQT基因表达受多种植物激素调控。为了进一步研究烟草绿原酸合成代谢相关机理，本项目拟以新克隆到的烟草HQT基因为基础，克隆其启动子序列，分析在不同植物激素处理下，HQT基因的表达模式以及绿原酸含量的变化；构建HQT基因过表达以及启动子截短的转基因烟草；并比较在不同激素处理下表型差异；结合RNA-Seq等技术，分析鉴定激素调控HQT基因表达信号转导途径关键基因及调控元件；探查植物激素调控烟草绿原酸合成的相关分子机制，为烟草绿原酸的合成调控研究提供新的思路。

Chlorogenic acid (CGA) in vitro has anti-cancer, anti-inflammatory, anti-bacterial, anti-virus and other extensive pharmacological effects. Tobacco is one of the plants with high chlorogenic acid content, which can extract large amount of CGA. It has been demonstrate that HQT gene regulates plant CGA synthesis, and the HQT gene expression is regulated by a variety of plant hormones. In order to further study the mechanism of CGA synthesis in tobacco, in this study, based on our former cloned HQT gene, the promoter of HQT gene would be cloned. The expression model of HQT and CGA content in tobacco were analyzed after different plant hormone treatments. By using bioinformatics analysis, the corresponding cis-element of the promoter related to plant hormone would also be characterized. Meanwhile, the significant differences between control and transgenic tobacco overexpressing HQT would be observed with the parameters of plant height and chlorophyll contents under different plant hormone treatments. Additionally, combining with RNA-Seq analysis of the differences between control and plant hormone-treated tobacco plants, the significant induction of genes involved in CGA synthesis would also be identified. These results would clarify the positive roles of HQT in CGA synthesis under plant hormone treatments and provide some new theoretic basis for the abiotic stress study.