近年来，食品中多种小分子环境内分泌干扰物（EDCs）的危害及滥用越来越得到全世界的高度关注，本课题针对国内外EDCs快速检测新技术缺乏的现状，以污染食品中典型EDCs（己烯雌酚、雌二醇、双酚A等）为研究靶标，建立以新型纳米磁性材料为介质的靶标诱导变构解离-指数扩增配体系统进化技术，开展EDCs的核酸适配体的差异和富集筛选；采用微量热泳动技术开展 EDCs 与其高亲和力核酸适配体分子间相互作用分析和亲和力机制研究；将适配体、纳米材料与低场核磁共振技术有机结合，研制EDCs 检测灵敏度高、特异性好的微型智能低场核磁共振生物传感器，建立一套基于高亲和力功能性核酸适体的食品中EDCs低场核磁共振生物传感痕量定量检测新技术。该课题是预防医学、生物学、物理学等多学科交叉的前沿性研究，其研究成果可用于食品安全监控、环境监测、出入境检验检疫等领域，对推动卫生分析化学新技术发展具有很好的理论价值。

In recent years, the hazard and abuse of a variety of endocrine disrupting chemicals ( EDCs ) in food has gotten more and more attention all over the world ,and then it is short of novel rapid detecting technologies on EDCs, So the object is focused on typical EDCs such as diethylstilbestrol(DES ) , 17-β-Estradiol ( E2 ) , bisphenol A(BPA) , etc. and ready to establish a new target-induced-structure-switching-dissociation systematic evolution of ligands by exponential enrichment technology for the preparation of high-affinity functionalized aptamer by discrepant and enrichment screening. Continuous studies of affinity mechanism for inter-molecular interaction between EDCs and their corresponding high-affinity aptamers are carried out employing microscale thermophoresis (MST) technology, the affinity and specificity are further improved. Then, via the optimization the reactive conditions of low field nuclear magnetic resonance (LF-NMR) detection system, LF-NMR technology based on high-affinity functionalized aptamer and nano-materials for the trace and quantitative detection of EDCs is established and the miniature intelligent LF-NMR biosensors is developed. Based on the above, further improve the performance of EDCs detecting sensitivity, specificity, detection range, etc. We combined the aptamer and LF-NMR to establish a detection technology with high affinity, good specificity via the detection of the typical model target–EDCs and achieve LF-NMR biosensor. It is advanced interdisciplinary studies on the series of novel technologies of Preventive medicine,Biology,Physics.These series of novel technologies will be applied in the fields of environmental pollution and risk of food safety assessment and control, and entry-exit inspection and quarantine, etc.,with theoretical significances and practical prospects, broader the development for hygienic analytical chemistry and related areas of novel detection technologies.