炼焦作业产生的焦炉逸散物中含有大量的多环芳烃（PAHs）和重金属等化学污染物，长期吸入可造成一系列健康损伤效应，最后引起多种疾病（如心血管疾病、呼吸系统疾病、肺癌等）的发生。本课题组于2010年建立了1628例焦炉工职业人群队列，并在2014年进行了随访。本项目拟在前期研究工作的基础上，将前瞻性队列研究和分子生物学研究相结合，通过检测队列人群基线和随访后焦炉逸散物主要成分（PAHs、金属）内暴露水平和多种早期健康标志物水平（如代谢紊乱、肺功能下降、氧化应激、DNA损伤、端粒长度缩短），采用GSA芯片对基因组遗传变异进行分型，建立焦炉逸散物主要成分单独和复合暴露与机体早期健康效应动态变化的剂量-效应关系，发现与焦炉逸散物主要成分在影响工人早期健康效应水平上具有交互作用的遗传位点，并阐明其生物学机制。本研究将为职业相关疾病发生机制的进一步阐明、疾病早期预警和职业健康监护体系的制订提供科学依据。

The coke-oven emissions, produced during the coking process, contains large amount of chemical pollutants, including polycyclic aromatic hydrocarbons (PAHs) and heavy metals. Long-termly exposed to high levels of PAHs and heavy metals can cause a series of health damage effects, leading to the development of many chronic diseases like cardiovascular disease, chronic obstructive pulmonary disease, and lung cancer. Our research group had set up an occupational cohort of 1628 workers from a coke-oven plant in 2010, and completed the first follow-up study of this population in 2014. Based on the previous research work, we aim to investigate the interaction effect and potential mechanism of PAHs and metals with genome-wide variations on the early health damage of the coke-oven workers in the present research project. We will detect the internal exposure levels of PAHs and metals by using GC-MS and ICP-MS systems, and measure the levels of several early health effect biomarkers, including metabolic disorder, lung function decline, oxidative stress, DNA damage, and telomere length shorten, both in the baseline and follow-up stage of this prospective occupational cohort. The genomic variations of all participants in this cohort is going to be genotyped by using Illumina Global Screening Array (GSA). We will evaluate the dose-response relationships between PAHs and metals exposure levels and change of early health effect, reveal the interaction effects of PAHs and metals with genetic variations on the early health effects among the coke-oven workers, and further explore the underline mechanisms in the followed molecular biological studies. The results of this project will help uncover the biological mechanisms of the occupational related diseases, and provide important clues to establish appropriate scheme of early occupational health surveillance for the coke-oven workers.