油菜倒伏常年多发，严重降低产量，影响菜籽品质。迄今仅限于形态、生理、生化、经典遗传、分子标记等不多的研究。开展油菜抗倒伏调控机理解析、功能基因克隆等研究意义重要且紧迫。鉴于抗倒性与维管束发育、次生壁加厚相关，而MYB转录因子主要影响次生壁加厚和木质素积累，研究主要内容有：1)基于电子克隆、器官组织间和抗倒/倒伏材料间的RT-PCR，以及根、茎秆切片RNA原位杂交，鉴定油菜根、茎秆中木质部优势或特异表达、与抗倒伏密切相关的候选基因1-2个；2)克隆目的基因全长，亚细胞定位后，构建超量表达和RNA干扰载体，遗传转化油菜并通过分子检测，花粉培养后获得纯合的目标基因表达的优株系；抗倒伏、抗病性状等功能验证，推测分子调控机理。3）酵母单杂交、双向电泳、质谱分析，进行蛋白质组水平研究，推测调控机理及抗病和抗倒关系。研究结果可为获得一条新的油菜高抗倒伏或抗病能力的路径提供有价值的信息。

The rapeseed lodging happens frequently, which not only reduces the yield, but also affects the seeds quality. Until now, some researches for the rapeseed lodging resistance were focus on the morphological, physiological, biochemical and molecular marker. It is very important for the rapeseed lodging research by means of molecular mechanism analysis, function gene cloning and so on. It is well known that the MYB genes associated with the rapeseed lodging resistance by effects the secondary wall thicken and lignin accumulation. Our research include: 1) Identify the root and stem specified or up regulated MYB gene by differential expression analysis among the different plant tissues or lodging/(lodging resistant) plants in Brassica napus. 2) Clone the full length target MYB gene, subcellular localization and construct the over-expression and RNA interference vectors to transform B. napus followed by the PCR, RT-PCR identification and pollen culture to select the homozygous transgenic lines. Identify characters of lodging and Sclerotinia sclerotiorm resistant of target genes, and speculate the molecular mechanism. 3) Study on binding proteins of target genes by yeast one hybrid, two-dimensional electrophoresis, mass spectrometer, and speculate the regulation mechanism and the relationship between lodging resistance and disease resistance. The results of this research provides valuable information to obtain a new path to improve the ability of lodging resistance or disease resistance of Brassica napus.