SNARE蛋白介导囊泡运输的膜融合过程，前人对植物SNARE蛋白参与胞内不同膜区室之间的分泌运输进行了深入研究。然而，SNARE蛋白在植物胞吞过程中的作用尚不清楚。研究表明，网格蛋白介导的胞吞是植物膜蛋白胞吞的主要途径。我们前期的研究结果暗示，SNARE蛋白VAMP721和VAMP722参与植物胞吞作用。为进一步揭示其参与胞吞的机制，本项目以拟南芥为材料，着重展开三方面的研究：(1)分析VAMP721和VAMP722在植物胞吞运输中的功能；(2)研究VAMP721和VAMP722与网格蛋白介导胞吞的相关性；(3)阐明VAMP721和VAMP722参与胞吞的作用机制。在本项目研究中，拟采用可变角度全内反射荧光显微术、荧光互相关光谱和FRET-FLIM等一些新技术、新方法，旨在揭示SNARE蛋白在网格蛋白介导的植物胞吞动态过程中的作用，为进一步研究植物胞吞机制和膜蛋白活性调控提供新的依据。

SNARE proteins mediate the membrane fusion during the process of vesicle trafficking. Previously, the investigations about the function of plant SNAREs mainly focus on the regulation of secretory trafficking within endomembrane system. However, the roles of SNARE protein in plant dynamic endocytosis are still unclear. In plants, clathrin-mediated endocytosis is the principal route by which cells internalize the transmembrane proteins. In the present study, we will investigate the function of Arabidopsis SNAREs VAMP721 and VAMP722 in plant endocytosis based on our new results by means of dynamic imaging in the living cells together with biochemic and genetic approaches. First, we will determine the roles of SNAREs VAMP721 and VAMP722 in plant endocytic process. Then, the correlation of the dynamics of VAMP721 and VAMP722 at plasma membrane with clathrin-mediated endocytosis will be analyzed. Finally, we will further explore the mechanism for VAMP721 and VAMP722 involved in clathrin-mediated endocytosis. In the course of the investigation, some new methods and techniques including variable angle total internal reflection microscopy, fluorescence correlation spectroscopy and FRET-FLIM, will be used in this study with the aim of uncovering the function of VAMP721 and VAMP722 in dynamic process of plant endocytosis. Taken together, the results of this study will provide new insights into the regulation mechanisms of clathrin-mediated endocytosis and membrane protein function in plant cells.