植物生长发育功能基因的解析是当今植物学研究的重点。在前期研究中，我们分离到一个拟南芥T-DNA插入突变体athspr。与野生型相比，该突变体植株矮化、各器官体积减小、细胞长度缩减，而过表达AtHSPR基因时则细胞长度明显增加，表明AtHSPR可能参与了细胞扩展的调节，但其调控机制尚不知晓。因此，本项目拟以该突变体、野生型和AtHSPR过表达系为材料，采用常规和多种细胞与分子生物学方法，比较分析各株系的表型和细胞结构差异；探究AtHSPR如何调控细胞骨架的形态结构；解析其是否参与了细胞壁的松弛和质子酸化；阐明AtHSPR如何与IAA27蛋白相互作用并参与生长素介导的细胞扩展；通过转录组学全面解析AtHSPR调控植物细胞扩展的靶点基因及调控网络，揭示AtHSPR在植物细胞扩展中的功能及其调控机制。显然，本项目将为揭示植物生长发育机理提供新思路和新途径，具有重要的科学意义和潜在的农业生产价值。

The study of functional genes on plant growth and development is one of current important and hot topics in plant biology sciences. In previous studies, we isolated a T-DNA insertion mutant in Arabidopsis, athspr (Arabidopsis thaliana heats shock protein related), which shows dwarf, decreased organ volume, and small cell size with shorten long axis, compared to wild type. However, the length of the cell is obvious increase in transgenic plants over-expressed AtHSPR gene, suggesting that AtHSPR may be involved in regulation of cell expansion, but its regulatory mechanism is unknow. In the present project, we plan to compare and analysis the phenotype and structure differences of athspr mutant, wild type and AtHSPR over-expression lines; to explore AtHSPR how to regulate cytoskeleton morphological structure and participate in auxin mediated cell extensions; to analyze whether AtHSPR is involved in cell proton acidification pathway; to explain interactions between AtHSPR protein and IAA27proteins, and to characterize all target genes of plant cell extension mediating by AtHSPR and it’s molecular regulation network at the transcription level, in order to reveal the regulatory function of AtHSPR in plant cell extension and signal transduction, using conventional, cell and molecular methodes. Obviously, the study of the function of AtHSPR will provide a new pathway and idea for revealing plant growth and development mechanism and it’s signal pathway. This project has not only the important scientific significance, but also the potential application in improving agricultural production.