坛紫菜是一种进化地位特殊的真核红藻，也是我国特有的人工栽培种，具有重要经济价值。前期研究发现，随着采收次数增加，养殖区域海水温度逐渐降低，同时不同类型糖含量与结构逐渐发生变化，揭示其体内必然存在某些保护性适应机制，以有效避免低温环境对其造成的损害。目前低温环境影响坛紫菜体内糖物质的生理调控机制尚不清楚。本项研究将依托于浙江坛紫菜养殖基地，以坛紫菜中糖代谢相关基因的发掘为主要目标，以低温胁迫处理叶状体为参照对象，通过转录基因组学分析技术，分别从糖成分与结构、细胞生理生化、分子转录水平探索坛紫菜细胞糖代谢主要转换机制之间的影响因素、糖分解后的二次碳源重新分配机制以及糖的分配与积累，发掘糖代谢与转运相关基因及其表达规律，揭示糖介导的坛紫菜抗低温应答机制。本项目的实施将为完善养殖坛紫菜品质评价体系奠定基础，丰富和拓展对光合生物碳代谢机制的认识，同时对坛紫菜的栽培实践和高值化利用有重要的指导意义。

Porphyra haitanensis is an evolutionally special eukaryotic red algae species which is specifically cultivated in China because of its economic importance. Previous studies found that when the number of times for harvest increased, the seawater temperature decreased and gradual alterations in the content and structure of various carbohydrates were observed, suggesting the existence of some adaptive mechanism prevent the red algae from the damage caused by low temperature. However, this potential mechanism remains unexplored. Taking advantage of the Zhejiang Laver Breeding Base, this study is aimed to explore the sugar metabolism-related genes in P.haitanensis. In our study, red algae samples harvested at different time periods will be subjected to cold stress and then transcriptome analysis, and in aspects of sugar content and structure, cellular psychology and biochemistry, and RNA transcription, we will explored the factors that influence the major conversion mechanism of carbohydrate metabolism decomposition, the post-sugar-decomposition redistribution mechanism of secondary carbon resource, and the sugar allocation and accumulation. Furthermore, the glucose metabolism- and transportation-related genes in the red algae and their expression patterns will be investigated in this study to reveal the sugar mediated protective mechanism responding to low temperature. This project will establish a foundation of quality evaluation for P. haitanensis breeding, expand the knowledge of the biological mechanism of photosynthetic carbon metabolism, and provide guidance for cultivation and high-value utilization of P. haitanensis.