肌醇是生物体必不可少的环状多元醇，其多种多样的衍生物参与了许多重要细胞过程。尽管在动物中有大量的研究，肌醇及其衍生物在植物细胞中的作用还有许多未知。申请人最近的研究表明，肌醇相关代谢途径对植物体细胞分裂有重要的作用。我们通过反向遗传学筛选得到的肌醇途径拟南芥突变体cp，表现出受抑制的根细胞分裂，部分细胞不能通过正常的细胞板生长完成胞质分裂而是形成中间空洞的细胞壁，以及发生内复制现象。然而，肌醇代谢途径是如何影响植物有丝分裂还不清楚。本项目拟结合肌醇衍生物处理、荧光标记示踪、化学抑制剂处理和构建EMS恢复突变体库等手段，揭示相应肌醇衍生物在拟南芥细胞板生长、细胞周期调控中的作用机理，并拓展对于植物细胞分裂精密调控机制的认识。预计本研究的结果可以用于实际生产中作物细胞分裂的调控，实现农作物产量的提高。

Inositol is an essential cyclic polyol in living organisms. Its diverse derivatives are involved in plethora cellular processes. Despite numerous discoveries in animal research, there are still many unknowns for the biological functions of inositol and its derivatives in plant cells. We recently found that inositol metabolic pathway is essential for plant mitosis. By reverse genetic screening, we obtained an inositol metabolism related Arabidopsis mutant cp, which shows inhibited root cell division, forms hollow cell walls through centripetal cell plate development, and displays endoreduplication. However, the link between inositol metabolism and plant mitosis remains elusive. Here we propose to employ inositol derivative treatments, fluorescent marker tracing, chemical inhibitor treatments, and EMS mutagenesis, etc, to reveal the mechanism of inositol derivative regulated Arabidopsis cell plate development and cell cycle, and advance our knowledge of precise regulation in plant cell division. The expected results of this proposal could also provide clues in cell division control of crops and improve the yield in agriculture practice.