植物生物钟参与环境适应性及诸多关键农艺性状的调控，深入解析生物钟转录和转录后的调控机制将为其在农业生产中的应用提供理论基础。植物生物钟早晨和傍晚蛋白复合体时空动态调控是目前研究的重点内容之一，蛋白复合体的时空动态调控与环境信号的周期性变化同步孵赋予了生物钟精细复杂的调控机制。为深入探究CCA1转录蛋白复合体的构成及作用机理，本课题执行以下研究内容：1、利用LC-MS等技术手段筛选鉴定CCA1转录复合体的新作用组分，对CCA1与其关联蛋白间时间依赖的动态互作特性进行解析；2、通过CCA1为核心的转录复合体相关突变体材料的转录组测序，分析不同时间/相位表达的功能转录复合体的靶基因，确定CCA1参与光周期开花时间以及夜间逆境胁迫应答的分子机理。预期研究结果将阐明CCA1转录因子参与生物钟与诸多昼夜信号应答的分子机理及生物学意义。

The circadian clock in higher plant contributes to fitness and multiple critical agronomic traits. Deep insight into the circadian mechanism of transcriptional and translational feedback loops aims to actively pursuing the use of clock modulation in agricultural production. Transcriptional complex functions spatiotemporally in response to the daily changes of environmental stimuli, and make it possible for organisms to synchronize the individual diurnal rhythms with accurate phases. On the basis of our findings, the main part of this grant proposal is defining the components and molecular mechanism of CCA1-associated transcriptional complex. We are screening the new components whereby liquid chromatograph mass spectrometers, and uncover the discrepancy of different transcriptional complexes which shown dynamic interaction with each other. At the same time, another goal of this proposal is to determine the pathways upstream of RNA-seq identified target genes expression which involved in the circadian modulation of photoperiodic flower timing and night-time stress tolerance. This information will be used in a model to predict the function and significance of CCA1-associated transcriptional complexes in circadian clock and various signaling responses.