研究表明油菜素内酯激素能促进植物籽粒发育，但分子调控机制仍不清楚。本课题组田间试验表明油菜素内酯能显著增加玉米籽粒长，粒宽和百粒重。因此，利用高通量测序技术，对玉米自交系Mo17授粉后10天的籽粒经油菜素内酯处理后进行转录组测序，测序结果进行差异表达基因筛选并进行转录因子预测，初步筛选到27个差异表达的籽粒发育相关功能基因，52个可能介导油菜素内酯调控籽粒发育的候选转录因子。本项目在此基础上，以这27个差异表达的基因为靶基因，进一步筛选介导油菜素内酯调控籽粒发育相关基因表达的转录因子，通过亚细胞定位，酵母激活，DNA结合实验，凝胶阻滞电泳，胚乳瞬时表达等方法对候选转录因子进行鉴定，对鉴定的转录因子构建植物表达载体，获得转基因植株，对其性状鉴定并通过染色质免疫沉淀技术分析转录因子的DNA结合原件及生物学功能，深入揭示油菜素内酯调控玉米籽粒发育的分子机制。

Brassinolide, the first brassinosteroid isolated and shown to have biological activity Brassinosteroids (BRs) are a class of polyhydroxysteroids. It can improve seed development of maize, Howerver, the molecular regulation mechansim is still unclear so far. In field trial, we found that Brassinolide can increase grain length, grain width andhundred-grain weight, and obviously enhance the production. The seeds 10 days after pollination separated maize inbred line Mo17 have been treated with 10nM Brassinolide at 28℃ for in dark for 6 and 12 hours respectively. The differential expression analysis of the RNA-seq data about maize seed transcriptome reveals that 27 grain-development-related genes and 52 transcription factors have been screened. We speculate that the expressions of these grain-development-related genes should be regulated by transcription factors induced by Brassinolide. Based on this The candidate transcription factors induced by Brassinolide involved in seed development would be screened by the co-expression analysis, and will be identified by using the technology of EMSA, Subcellular localization, Yeast one-hybrid library and endosperm transient expression system. And then, the biology function of transcription factor will be researched by transgene maize. The results would reveal the molecular mechanisms of seed development regulated by Brassinolide.