细菌抗生素耐药已成为目前威胁人类健康的全球性公共卫生问题，目前用于临床的主要抗生素以传统的抑菌/杀菌机制已不能有效地杀灭频繁出现的多重耐药细菌和泛耐药细菌。因此，新型抗菌药物的研发迫在眉睫。申请人在研究碳纳米材料的过程中发现所制备的多氮掺杂碳量子点（mN-CQDs）对大肠杆菌和金黄色葡萄球菌均有一定的抑菌作用。预实验结果显示mN-CQDs表面基团电荷不影响其荧光强度，会影响其抗菌活性。为了阐明mN-CQDs的抗菌特点和生物特性，本项目将确定mN-CQDs表面的功能基团和mN-CQDs的抗菌谱，研究mN-CQDs的体内外抗菌活性及其最小抑菌浓度，并探讨mN-CQDs可能的抗菌机制，研究mN-CQDs的细胞毒性和对生物体的损伤，以及mN-CQDs在体内的吸收、分布、代谢和排泄等变化规律。为解决细菌耐药和抗菌药物研发提供新的思路和策略，并为mN-CQDs在体内的应用提供可靠的实验数据和研究基础。

Bacterial antibiotic resistance has become worldwide public health problem threat to human health. At present, the main antibiotics in clinical the traditional antibacterial/sterilization mechanism cannot effectively kill multiple drug-resistant bacteria and extensive drug-resistant bacteria. Therefore, the research and development of new antibacterial drugs is impending. We found the prepared more nitrogen doped carbon quantum dots (mN-CQDs) have certain bacteriostatic effect on Escherichia coli and Staphylococcus aureus. Preliminary experimental results showed that the surface charge of mN-CQDs does not affect fluorescence intensity, but affect antibacterial activity. To illustrate the antibacterial characteristics and biological characteristics of mN-CQDs, the project will determine the surface functional groups and the antibacterial spectrum of mN-CQDs, research the antibacterial activity and minimum bacteriostasis concentration of mN-CQDs in vivo and in vitro, investigate the antibacterial mechanism of mN-CQDs, pinpoint cell toxicity and damage for the organism of mN-CQDs, and study absorption, distribution, metabolism and excretion of mN-CQDs in vivo, etc. To solve the problem of bacteria resistance and development of antibacterial drug, the project provides new ideas and strategies, and reliable experimental data and research basis for mN-CQDs in vivo.