石蒜科植物特有的生物碱加兰他敏在临床上被广泛用于治疗阿尔茨海默病。加兰他敏生物合成途径已基本明确，但其转运模式仍有待阐释。前期，申请人在石蒜属植物忽地笑差异转录组数据中，发掘到一个ABC类转运蛋白LaMRP1，初步研究结果表明该蛋白极有可能参与加兰他敏的转运。为了探讨LaMRP1在加兰他敏跨膜转运中的作用，本申请拟通过酵母突变体功能互补、膜微囊和ITC等技术手段，进一步明确LaMRP1对加兰他敏的转运特征（转运方向、底物专一性和结合系数）；利用病毒介导的基因沉默（VIGS）技术抑制LaMRP1表达，检测植株各组织加兰他敏含量的变化，并结合LaMRP1的亚细胞定位和组织定位，从细胞、组织和植株三个层面揭示LaMRP1在加兰他敏转运过程中的作用，探讨加兰他敏在忽地笑体内运输的分子机制。研究结果将丰富生物碱在植物体内运输的分子机理研究，也为培育高加兰他敏含量的忽地笑品种提供一定的理论指导。

The alkaloids galanthamine extracted from Amaryllidaceae, has been used clinically for the treatment of Alzheimer disease. The synthesis pathway of galanthamine had been defined, but the research of transport and accumulation mechanism of galanthamine has not been reported yet. One ATP-binding cassette (ABC) transporter LaMRP1 was isolated from the comparative transcriptome analysis in response to MeJA treatment. The pre-experimental results showed that LaMRP1 was probably involved in the galanthamine transport. In this application, the function of LaMRP1 in galanthamine transportation would be charactcterized by using the yeast mutant strain, membrane vesicles and ITC. The change of galanthamine content would be tested after the expression of LaMRP1 was reduced by using the virus induced gene silence (VIGS). Combining the subcellular localization and the expression pattern of LaMRP1, this study would dissect the function of the LaMRP1 in galanthamine transmembrane transport and the long distance transpotation by evidences from three levels the cellular, tissue and the whole plant. This study will enrich the theory of plant alkaloid transport and accumulation mechanism. This application would provide a theoretical basis for genetic engineering to increase the content of galanthamine in Lycoris aurea.