立足于我国北方春玉米区低温限制还田玉米秸秆当季高效腐解的实际问题与技术需求，在课题组筛选驯化获得的玉米秸秆低温高效降解复合菌系GF-20（10℃，降解率32%）的基础上，针对进一步提高其玉米秸秆降解效率的核心问题，以揭示GF-20微生物组成结构为切入点，以明确其菌种间协同关系为突破，研究GF-20降解玉米秸秆微生物群落结构时空演替规律，并分离纯化单菌株，复配功能单菌，检测其稳定性和秸秆降解效率等，利用“Knock out”设计明确各菌种间的互作关系，揭示GF-20玉米秸秆低温高效降解生理机制，构建菌种模型，为进一步探索组配低温高效玉米秸秆降解复合菌系提供理论支持。

The cool climate limits efficiency decomposition of returning straw in spring maize region of north China is urgent problems in theoretical and technical area. In order to promoting efficient degradation of corn stover in low temperature regions, the before study a microbial consortium with efficient corn stover degradation ability at low temperature (the stover degradation ratio was 32% under 10℃ cultivation condition) was screened out by restrictive cultivation and cold acclimation and then it’s growth characteristics, diversity of microbial composition and stability were identified. Our present study takes clarifying the microbial composition structure of consortia GF-20 and it’s intertwined interspecies relationships as the breakthrough point, to further improve the corn stover degradation efficiency. Composite microbial system GF-20 was continuously subcultured under 10℃, the temporal-spatial succession of microbial community structure will be determined, and the key functional strains will be recombinant by "knock out" method, and its stability and the straw degradation efficiency will be tested so as to untangle the microbial synergistic network relationships between the strains of microbial consortium GF-20, furthermore, the physiological mechanism of the degradation of corn stover with high efficiency under low temperature will be evaluated. The result of this research will provide theoretical and technical supports for further explore ways of construction more efficient degradation microbial community.