高度近视晚期常见后极部视网膜萎缩致视力严重受损，与视网膜光感受器凋亡密切相关，目前尚无有效治疗方法。中医整体与局部辨证认为肝肾亏虚为其基本病机，临床观察证实肝肾同补之补精益视片（中医眼科名家陈达夫所创）明显改善高度近视视功能，对近视动物视网膜凋亡因子bcl-2及caspase3显著干预。故提出“肝肾同补通过调控HA/CD44-miR21-Bcl2/IAP信号通路改善实验性高度近视光感受器凋亡”的假说。采用肝肾同补法干预实验性高度近视小鼠，以HA/CD44信号通路为核心，通过mfERG检测、Tunnel染色、免疫组化检测、免疫印迹法、qPCR、转录组及miRNA测序等技术对光感受器及相邻的RPE、脉络膜血管内皮细胞和巩膜成纤维细胞进行检测，从功能、细胞、蛋白到基因来探明肝肾同补调控光感受器凋亡延缓视网膜萎缩的靶点及分子机制，为中医早期干预提供实验支撑和科学依据，丰富“既病防变”的预防学思想。

In the late period of high myopia, retinal posterior atrophy connected with retinal photoreceptor apoptosis often cause serious visual impairment, and there is no effective treatment currently. Integral and local syndrome differentiation of Traditional Chinese medicine (TCM) considers that such pathologic change relates to the deficiency of liver and kidney. Through clinical observation, we have found that the Bujingyishi tablet which has the effect of tonifying liver and kidney, can regulate and control the visual function of patients having high myopia, and has also the significant intervention effect on the bcl-2 and caspase3 factors causing apoptosis of the retina of animals having myopia. So we put forword hypothesis that“The method of tonifying liver and kidney to regulate and control the apoptosis of experimental high myopia retinal photoreceptor through the signaling pathway of HA/CD44-miR-21-Bcl2/IAP.Using the method of tonifying liver and kidney to intervent experimental high myopia C57BL/6Jmice, HA/CD44-miR-21-Bcl2/IAP signaling pathway as the core, and to carry out mfERG testing, Tunnel staining, western blotting, immunoblotting technique, qPCR, transcriptome sequencing and miRNA sequencing and so on to test the photoreceptor and its adjacent retinal pigmental epithelium(RPE), choroid vascular endothelial cell and sclera fibroblast, and to verify the effect of the target and molecular mechanism of tonifying liver and kidney to regulate and control the apoptosis of retinal photoreceptor through the joint analysis of the function, cell, protein and gene. The purpose of this research is to provide the experimental and theory basis for guiding the clinical early intervention of high myopia, rich the theory of prevention of “preventing disease from exacerbating”.