北细辛是东北道地大宗中药材，为阴生植物，挥发油是其主要有效成分。研究发现，与人参、西洋参等阴生植物相比，北细辛对光照需求有新特征，在北细辛生育期，梯次增加光照强度甚至给予全光照，挥发油含量呈明显递增趋势。北细辛挥发油90%以上成分是苯丙烷类化合物，是光合产物经莽草酸和肉桂酸路径合成的。项目通过研究（1）不同光照强度条件下，挥发油生物合成关键前体莽草酸、分支酸、苯丙氨酸、肉桂酸含量和催化反应的酶DAHPS、CM、PAL、C4H、4CL的活性；（2）DAHPS、CM、PAL、C4H、4CL体外单独表达时，对强光辐射的应答；（3）强光辐射对酶转录因子表达的影响及光信号转导因子对酶合成的影响。明确强光辐射对DAHPS、CM、PAL、C4H、4CL表达的调控；光信号转导因子、转录因子对强光辐射的应答以及在挥发油合成中的调控功能。在光生理和分子水平上揭示光强调控北细辛挥发油生物合成机理。

As a shade plant, the Asarum heterotropoides Fr. Schmidt Var. mandshuricum(Maxim.) kitag. is the trueborn herb in the Northeast China and volatile oil is its main active constituent. The previous research showed there was difference between A. heterotropoides Var. mandshuricum and Panax ginseng,Panax quinquefolius which were also shade plants on photosynthetic characteristics. The content of volatile oil in the hair roots exhibited graded changing trend with the light irradiation which was the light environment of the Asarum heterotropoides Var. mandshuricum growing in increasing from low light to full sunlight gradually. And, this result was inconsistent with most shade plant secondary metabolism products biosynthesis characteristics. The A.heterotropoides Var. mandshuricum volatile oil consists of phenylpropanoid compounds mainly and was synthesized by the shikimate pathway and cinnamate pathway. The objective of current proposal aim to study the synthesis of shikimate, chorismate, phenylalanine and cinnamate, the activity of DAHPS,CM,PAL,C4H and 4CL in plant tissue and single enzyme expression in vivo in different light irradiation, the expression of light signal transduction factor and transcription factor in high light. The expected results will enable us to explain how the high light influences the activity of DAHPS, CM, PAL, C4H and 4CL respectively and the regulation function of light signal transduction factor and transcription factor in the process of volatile oil biosynthesis.