本项目以研究温度对黄鳝亲本培育、产卵、孵化和苗种培育的影响为基础，利用生理生态学、内分泌学和分子生物学技术，研究温度对黄鳝性腺发育过程中血清雌二醇和睾酮含量以及性腺指数影响的变化规律，观察温度对黄鳝性腺发育的影响以及由雌性生殖细胞转为雄性生殖细胞的转化过程；并分别对雌雄黄鳝的性腺组织取样，提取RNA进行转录组测序，通过表达谱筛选出差异基因，挑选关键基因进行功能研究，确定关键基因对性别转化的调控作用，以阐明温度对黄鳝性别调控的分子机制。本项目针对黄鳝怀卵量小和天然性逆转的特性，结合前期营养条件、养殖密度和外源性激素等调控性别的研究基础，通过温度这一影响黄鳝性腺发育和性别分化的关键因子，探索黄鳝性别分化发生机制，可为深入阐明性别分化机制和全面攻克黄鳝人工繁殖技术提供新的理论和方法，实现黄鳝苗种的批量生产，彻底解决养殖中苗种短缺的瓶颈问题。

This project takes advantage of the physiological ecology, endocrinology, as well as the molecular biology technology to study how temperature affects serum estradiol, testosterone and gonadosomatic index during the gonad development process, and observe the temperature's effect on gonad development as well as how the female germ cell transfers to the male germ cell in the rice field eel. All of these were based on the temperature's effect on parent breeding, spawning, hatching and fry rearing in the species. And then sample the male and female gonad tissue, transcriptome sequence the extracted RNA, sift the differential genes via expression profile, study the key genes to ensure them regulation effect on sex reversal and clarify the molecular mechanisms in rice field eel. Aiming at the special characteristic of low fecundity and natural sex reversal, the effects of lots of factors, such as nutritional condition, stocking densities and exogenous hormones on controlled artificial multiplying and sex regulation were researched and some achievements were made.According to previous experiments, this project makes use of the temperature, the key factor that affects gonad development and sex differentiation, to explore sex differentiation mechanism, which can clarify the sex differentiation mechanism and provide new method and theory for artificial multiplying technology, then the quantity production can be achieved and the problem of fry's shortage can be solved completely in this species.