阿是穴的临床应用广泛，但对其治病机制的研究不足。目前，干针疗法对针灸理论形成了一定的冲击，这与阿是穴研究不被重视密切相关。以往常用局部的无菌性炎症解释阿是穴的形成，但刺激阿是穴后局部穴位的致炎致痛物质的改变及其神经内分泌机制还不清楚。本项目拟围绕刺激阿是穴治疗肌筋膜病的疗效与下丘脑-垂体-肾上腺的激活反应之间的相互关系展开研究。通过对临床上常见的肌筋膜病(第三腰椎横突综合征)局部病理组织及皮肤进行观察，采用组织化学定位、分子生物学分析定量的研究方法，明确阿是穴刺激在损伤局部无菌性炎症发生过程中引发的HPA激活所产生的神经-内分泌-免疫物质的级联反应；进一步通过对HPA轴激活的全身效应的观察，研究阿是穴产生治疗效应的神经内分泌学机制；并对针、灸刺激所产生的疗进行比较。为阿是穴刺激治疗肌筋膜病提供科学依据，回应国际上针灸理论面对干针疗法的挑战，为临床运用阿是穴奠定科学基础。

The clinical application of Ashi point is widespread, but the research on the mechanism of Ashi point was obvious insufficient. At present, the dry needle therapy has formed a certain impact to the traditional acupuncture and moxibustion theory; this is closely related to the ignored research of Ashi Point. A local aseptic inflammation commonly used to expainle the formation of Ashi point.But after stimulation, the change of the material in local inflammatory pain and the neuroendocrine mechanism is not clear. This project intends to focus on the relationship between the curative effect of acupuncture point and the activation of the hypothalamus - pituitary - adrenal axis.Through the clinically common disease (the third lumbar transverse process syndrome) local pathological tissue and skin, using histochemical localization, the analysis of quantitative research methods of molecular biology, observing adrenocorticotropic hormone releasing hormone releasing in the process of damage local aseptic inflammation in Ahi point stimulation, and resulting HPA activation in local. Further through the observation of the systemic effects of HPA axis activation, study the neuroendocrinology of Ashi point has treatment effect mechanism. Through the comparison of the acupuncture and moxibustion effect in therapy, to provide the scientific basis of the acupuncture point stimulation in the treatment of myofascial disease and response to acupuncture and moxibustion theories on international to face the challenge of the dry needle therapy.