蚜虫在受到外界威胁时，从腹管中分泌报警信息素使周围的同伴能够迅速逃避敌害。在害虫防治的应用上，这种报警信息素不仅可以有效防止蚜虫聚集、控制蚜虫数量，而且为天敌搜寻蚜虫提供了可靠的线索。因此，蚜虫报警信息素类似物的合成和应用一直是防治蚜虫危害进而减少植物病毒传播的热点课题。通过近20年的研究，虽然对报警信息素引起的蚜虫的生理行为及触角电位反应的研究取得了重要进展，但是对蚜虫识别报警信息素的分子机制却有待深入研究。本研究采用比较基因组学、分子生物学和电生理学等方法，鉴定和克隆编码蚜虫报警性信息素的气味受体和气味结合蛋白基因；通过体外表达的方法重建蚜虫报警信息素识别系统，阐明蚜虫识别报警信息素的神经和分子基础，为从分子水平筛选和设计稳定的、不易挥发的蚜虫报警信息素类似物，进而用于控制蚜虫危害提供理论依据。

When aphids are attacked, alarm pheromone secreted from their cornicles or siphunculi enables neighbors to escape damage. The aphid alarm pheromone could be used in direct application treatments to crops to reduce aphid colonization and also provide reliable cues for host-seeking of parasitoids or predators. Synthesis and application of alarm pheromone analogs have been a hot topic of how to control aphid damage and further to reduce virus spread. Although researches in the past decade made important progress in physiology and behavior of aphids raised by alarm pheromone, the molecular mechanism of alarm pheromone-encoding in aphid needs is largely unknown. In this proposal, Odorant receptor and odorant binding protein genes encoding aphid alarm pheromone will be identified and functional characterized through comparative genomics, molecular biology and electrophysiology. Based on this, we could reconstruct aphid alarm pheromone perception pathway in heterologous expression system and fruit fly mutant to elucidate the cellular and molecular basis of alarm pheromone preception, which may provide the logic proofs for screening and designing stable, nonvolatile analogs of aphid alarm pheromone, and further use to reduce aphid damage in the field.