番茄果实成熟涉及一系列复杂的生理生化代谢过程，并且是通过多个基因的精细调控所实现。这些基因表达调控主要有转录水平调控和转录后水平调控两种形式，并且转录因子和microRNAs分别是它们研究的热点。本项目拟通过研究番茄果实成熟过程中已知关键转录因子（RIN、CNR、NOR、TAGL1、AP2a、FUL1、FUL2、MADS1）所调控的microRNAs、其他转录因子、成熟相关功能基因，采用RNA-seq、small RNA-seq、降解组分析、EMSA、ChIP-PCR、WGCNA分析等技术建立番茄果实成熟的转录因子和microRNAs协同调控网络，明确重要转录因子和microRNAs调控果实成熟功能基因表达的协同调控途径，为全面、系统阐明果实成熟过程中功能基因表达调控机制奠定基础，并为培育耐贮藏、高品质的番茄果实提供新的思路。

Tomato fruit ripening involves a series of complex physiological and biochemical metabolism process, which was implemented by accurate regulation of multiple functional genes at transcriptional and post-transcriptional level. This project is proposed to construct synergistic regulatory network of tomato fruit ripening-related transcription factors and microRNAs through interaction relationship and coexpression relationship among microRNAs, transcription factors and ripening-related functional genes, which was regulated by the known key transcription factors (RIN, CNR, NOR,TAGL1, AP2a, FUL1, FUL2, MADS1) in the process of tomato fruit ripening. The purpose of this study is to clearly definite the synergistic regulatory pathway of controlling the fruit ripening-related genes expression by key transcription factors and microRNAs with the methods of RNA-seq, small RNA-seq, degradome analysis, EMSA, ChIP-PCR, WGCNA analysis, etc, which help to comprehensively and systematically clarify the regulation mechanism of functional gene expression in tomato fruit ripening, and provide new ideas of develop more storable and high quality tomato fruits.