采后病害导致桃严重损失。模式植物中亚硝基谷胱甘肽还原酶（GSNOR）可通过调控亚硝基硫醇的转移参与多种防御反应，GSNOR是否在诱导桃果实抗病中有重要作用，国内外鲜见报道。我们前期研究证实，NO可诱导桃果实提高抗病性，并检测到蛋白亚硝基化修饰现象。由此推测GSNOR及其介导的蛋白亚硝基化修饰可能在诱导桃抗病中起重要作用，因此，本项目拟利用激光共聚焦扫描显微镜和LC-ES/MS技术研究桃果实抗病中GSNOR的动态变化及与亚硝基硫醇、NO的关系；研究受GSNOR调控的诱导抗病途径关键因子的变化；通过蛋白组学、SPR-MS技术研究诱导抗病中GSNOR介导的亚硝基化靶标蛋白、作用位点和肽段序列；通过蛋白质互作研究抗病途径亚硝基化修饰对关键转录因子结构和DNA结合活性的影响，以期揭示GSNOR及其介导的蛋白亚硝基化修饰在NO诱导抗病中的作用机理，为探寻诱导采后果实抗病性的新方法提供理论基础和新思路。

Postharvest diseases in peach fruit cause great loss. Induced disease resistance is a kind of effective and promising method in controlling these diseases. More and more research in mode plants indicated that S-nitrosoglutathione reductase (GSNOR) was part of defense responses of plant by regulating the level of S-nitrosothiols (SNOs). Our previous study showed that nitric oxide (NO) could induce disease resistance of peach fruit, and several S-nitrosylated proteins were detected by surface plasmon resonance (SPR) and mass spectrometry (MS). Based on that evidence, this project is intended to (1) investigate the dynamic change of GSNOR and its relationship with endogenous NO and SNOs by the techniques of liquid chromatography- electrospray/MS (LC-ES/MS) and confocal laser scanning microscopy; (2) determine the key agents and gens in signal pathway regulated by GSNOR by molecular biology methods; (3) analyze the S-nitrosylated proteins mediated by GSNOR, target sites and peptide sequences of S-nitrosulated modification in proteins during the pathway of induced disease resistance by proteomics and SPR/MS techniques; (4) study the effects of S-nitrosylation or denitrosylation reactions on the structures of key transcriptional factors and their bonding activities of DNA by analyzing the interactions between DNA and protein or protein and protein. The mechanisms of the role of GSNOR and protein S-nitrosylation modification mediated by it in disease resistance induced by nitric oxide in peach fruit will be deeply elucidated by the research. The successful conduction of this project will be important for providing theoretical foundation for the application of new elicitors of induced disease resistance in postharvest fruits.