真核生物细胞核内非随机的染色质构型已经被证实参与各种细胞生理活动。基于高通量二代测序基础上的Hi-C染色质构型分析技术已经广泛应用于动物细胞核染色质构型分析。然而，植物上目前只在双子叶模式拟南芥中有相关的研究报道。本研究拟利用细胞遗传学观察和Hi-C染色质构型分析技术，分析、验证和比较水稻(日本晴)及其DNA甲基化met1-2突变体的细胞核染色质构型的模式和特征；在搜集和产出的各类基因组遗传结构和表观遗传修饰的高通量数据基础上，采用各种相关生物信息学数据处理和统计学检验的方法，探讨相应材料内Hi-C染色质构型特征与基因组遗传结构和表观遗传修饰间的可能关系，丰富和完善植物染色质构型的理论和模型。

Eukaryotic nuclear chromatin architecture existing in non-stochastic pattern has been confirmed to be involved in types of cellular activities. Hi-C chromatin structure analyzes are based on high-throughput Hi-C next generation sequencing and have been widely applied in animal model species. However, except those studies in Arabidopsis thaliana, systematic chromatin structural analyzes in other plant species, especially in crops, are still unexplored so far. Within current study, by adopting the classical cytogenetic observation and Hi-C sequencing,we proposed to analyze, testify, and compare the Hi-C chromatin structures in Oryza sativa L. ssp. japonica and its epigenetic met1-2 mutant in DNA methylation. Being dependent upon collecting public and generating own genetic and epigenetic modification data, further exploration about the relationship between the nuclear chromatin structure and aforementioned genetic structures and epigenetic modifications will be completed by using various approaches in bioinformatics and statistics, which will compensate and improve the theoretical models about nuclear chromatin structure in plant species.