作为细胞中丰度最高的非编码RNA，核糖体RNA(rRNA)是核糖体的重要组成部分，在核糖体的生物合成和蛋白翻译过程发挥着至关重要的作用。rRNA 的加工、修饰和成熟过程与核糖体的生物合成紧密联系，异常的rRNA 加工和修饰会干扰核糖体生物合成，影响生物体发育。相对于酵母和哺乳动物中的研究，高等植物中对于rRNA 的加工和修饰过程的认识才刚刚起步，其在植物生长发育过程中的功能和分子机制亟待阐明。本项目将围绕高等植物中核糖体RNA的加工和修饰这一关键科学问题，以参与调控核糖体RNA前体加工的拟南芥蛋白精氨酸甲基转移酶AtPRMT3和参与调控水稻温度响应的假尿苷合酶OsPUS1为切入点，阐明AtPRMT3参与调控核糖体RNA前体加工及植物生长发育的分子机理，揭示水稻假尿苷合酶OsPUS1参与RNA (特别是非编码RNA)假尿苷修饰、调控植物生长发育及响应温度变化的基因调控网络和分子机理。

As the most abundant non-coding RNA, ribosomal RNA (rRNA) is the fundamental component in ribosome, and plays essential roles in ribosome biogenesis and translation. The processing and modifications of rRNAs are tightly coupled with ribosome biogenesis, and abnormal processes will disturb the organism development. However, the biological functions and mechanisms of the rRNA processing and modifications in plants are infrequent and urgent to be investigated. In this project, we will mainly focus on the functional analysis of pre-rRNA processing and modifications. We will dissect the molecular mechanism of AtPRMT3 in pre-rRNA processing and plant development, and elucidate the regulatory network of OsPUS1 in pseudouridylation on RNAs (especially for non-coding RNAs) and plant development in response to temperature alterations.