刺萼龙葵(Solanum rostratum)是一种高度危险的有毒有害杂草，是我国重点管理的外来入侵物种，种子休眠的调控是遏制其种群萌发定植和传播扩散的关键途径。本研究重点围绕休眠特异相关基因DOG1（Delay of Germination 1）对刺萼龙葵种子休眠的调控这个主题，应用种子生态学、生理学和分子生物学的理论和方法，系统研究DOG1基因对刺萼龙葵种子季节性休眠的调控机制。主要对刺萼龙葵DOG1基因进行克隆和功能鉴定，研究DOG1基因的表达特性和时空特征、季节性休眠过程中种子萌发水平与DOG1基因表达水平的相关性、DOG1基因对环境信号的响应模式及与ABA和GA信号通路相关基因的互作机理，从而揭示DOG1基因参与调控刺萼龙葵种子季节性休眠的分子机制。研究结果不仅可以明晰DOG1关联的种子休眠调控信号通路，而且可为刺萼龙葵防控策略的制订提供重要理论依据，保障我国生态安全和粮食安全。

Buffalobur (Solanum rostratum Dunal) is a highly dangerous noxious and worst weed. It is an important invasive species for management by our government and now it is entering a rapid dispersal period characterized by broad occurrence, severe infestation and fast spreading. Regulation of the seed dormancy is the crucial strategy to control its further infestation and dispersal. Targeting on the main objectives of DOG1 (Delay of Germination 1), a dormancy-specific gene, for regulating seed dormancy of buffalobur and by using the theories and methods of seed ecology, physiology and molecular biology, mechanisms of DOG1 in regulating seasonal seed dormancy of buffalobur will be studied systematically. The research mainly deals with the cloning and functional analysis of DOG1 gene of buffalobur; the spatial and temporal expression of DOG1; correlation of DOG1 expression with the seed germination under seasonal dormancy process; the mode of DOG1 responses to environmental signals; the interaction of DOG1 with the genes related to GA and ABA biosynthesis pathway, and finally revealing the molecular mechanism of DOG1 in regulating seasonal seed dormancy in buffalobur. Our research not only could elucidate the DOG1 involved signaling pathway, but also could provide theoretical basis for making strategies for control of buffalobur, which will be helpful to safeguard the ecological safety and food safety of our country.