新疆世居的维吾尔族和哈萨克族人数较多具有不同于汉族的遗传特征、饮食生活习惯，极少与其它民族通婚，保持着较为单一的遗传背景，并且维吾尔族为冠心病的高发人群，为研究不同民族冠心病的遗传机制提供了良好的条件。我们对新疆不同民族健康成年人脂肪激素ASP水平的研究表明，民族间ASP水平存在差异，前期研究也显示ASP基因多态性在不同人种中均与甘油三酯水平有关，并且参与机体糖脂代谢的ASP基因多态性与新疆不同民族冠心病发病的异质性密切相关。本项目采用病例对照研究分析ASP基因多态性与新疆不同民族冠心病发病的关联；利用时实荧光定量PCR技术、蛋白印迹技术及免疫细胞化学法研究ASP基因敲除小鼠及对照组小鼠主动脉组织及细胞中的表达情况；利用RNA干涉技术联合DNA微阵列技术检测ASP基因沉默后细胞中基因表达谱的变化，从而找出与ASP基因相关的基因改变，阐明其在不同民族冠心发病异质性中的作用及机制。

Compared with Han population, Uygur and Kazakh have the difference in gene background, diet habits and lifestyle, and rarely intermarry with other ethnic groups in Xinjiang, witch formed a relatively pure genetic background. Investigations show that Uygur has a higher prevalence of CHD. This phenomenon above can create favorable conditions to research the genetic mechanism of CHD in different ethnicities. The levels of ASP is diverse among various ethnic healthy adults, and early study results indicated that the polymorphism of ASP gene was closely related to the levels of TG regardless of race, also, preliminary work showed that ASP gene involved in the glucolipid metabolism was strongly linked to the heterogeneity in different ethnic groups of CHD in xinjiang. This project adopts the case-control method based on the candidate gene strategy analyze the relevance and difference of ASP gene polymorphism among various ethnic groups of CHD, and we analyze ASP gene knockout mice and the control the expression of aorta tissue and cells using real-time PCR, Western blot and immunocytochemistry technology, furthermore, after silencing the expression of ASP gene using RNA interference technology, we detect the cell changes in gene expression profile exerting DNA microarray technology, witch try to find out the changes of related genes with ASP. Sequentially, To clarify the role and mechanism of ASP gene in the heterogeneity of CHD among various ethnicities.