非编码mRNA对基因功能的调控是生命科学领域最近兴起的研究热点。申请人已证实亚洲镰孢菌（Fusarium asiaticum）和灰葡萄孢菌（Botrytis cinerea）β-微管蛋白基因对多菌灵的药敏性和抗药性及在细胞周期中的部分功能是由内含子介导表达的。本项目拟以遗传背景比较清晰和易实验操作的亚洲镰孢菌为研究材料，以β1-微管蛋白基因的3个内含子及丢失的3个内含子和β2-微管蛋白基因的6个内含子为研究对象，对各内含子非编码RNA的生物合成、对宿主基因的药敏性/抗药性及在细胞周期等生命活动中的生物学功能调控进行研究，解析内含子非编码RNA对宿主微管蛋白基因的转录、翻译功能的调控机制，揭示内含子在真核细胞基因功能表达中的作用。其成果不仅有助于发现新的生命功能分子元件，而且对以特异性内含子非编码RNA为药靶研发选择性高效农用杀菌剂和抑制细胞分裂的抗癌药物及辅助药物也具有重要的指导意义。

Regulation of noncoding mRNA to gene function has become a research focus in life science recently. We have confirmed that the introns of the β-tubulin in Botrytis cinerea and Fusarium asiaticum are involved in the regulation of MBC sensitivity and resistance and part of life activities in cell cycle. This project intends to study the three introns in β1-tubulin and the six introns in β2-tubulin of Fusarium asiaticum which has clear genetic background and easy experimental operation, to analyze the regulatory mechanism of intronic ncRNAs in β1-tubulin and β2-tubulin on sensitivity and resistance to tubulin inhibitors and cell cycle, revealing the function of introns in in gene expression in eukaryotic cells. The project will not only help to discover new functional molecular components, but also be of great theoretical significance and practical value to develop novel high-efficient agricultural fungicides targeting intronic ncRNAs and anticarcinogens or ancillary drugs inhibiting cell division.