交泰丸对心肾不交型失眠疗效确切，但疗效特点不明晰，优势不突出，也缺乏相关神经机制的探讨。联合PSG和MRS，检测心肾不交型失眠患者口服交泰丸后睡眠时间和质量及脑内GABA的变化，HPLC和ELISA检测血中腺苷和褪黑素的含量。PCPA法建立心肾不交型失眠大鼠模型，对照唑吡坦，睡眠生物系统记录72h并比较wake、NREM和REM量及状态改变，解析交泰丸影响睡眠时间和质量的特点。分离海马、丘脑、脑干、皮质，UHPLC-MS/MS检测腺苷和褪黑素含量，Real-time PCR和Western bolt检测A1R、A2AR、MT1R、MT2R mRNA和蛋白含量，MRS检测GABA和Cr含量。临床与动物研究结合，明确交泰丸缓解心肾不交型失眠各症状的特点，确定改善入睡困难的优势疗效。微观递质和宏观活动结合，从基因、蛋白和代谢组，阐明交泰丸在内稳态、主时钟和觉醒抑制方面的睡眠诱导机制和脑区相关性。

Jiaotaiwan has definite efficacy on insomnia of non-interaction between heart and kidney.However the therapeutic effect is not clear,the advantage is not outstanding, and it is also lack of discussion of related neural mechanism.Combined PSG with MRS to test sleeping time,quality and variation of GABA in brain after englobement of Jiaotaiwan in patients with insomnia of non-interaction between heart and kidney.HPLC and ELISA detect contents of adenosine and melatonin.Use PCPA method to establish insomnia rat model of non-interaction between heart and kidney,and controled with zolpidem,sleeping biological system recorded and compared in 72h as follows:the amount and state change of wake,NREM and REM;to analyze characters of influence on sleep time and quality.Isolate hippocampus,thalamus,brainstem and cortex.UHPLC-MS/MS examine the quantities of adenosine and melatonin.Real-time PCR and Western bolt test contents of A1R,A2AR,MT1,MT2,mRNA and protein.MRS detect contents of GABA and Cr.Combined with clinic and animal study,we aim to clear feature of remission of kinds of symptoms of insomnia of non-interaction between heart and kidney,so we can definite advantage efficacy of improving difficulty falling asleep.Based on micro transmitter and macro activity,and from gene,protein to metabonomics,we explored the possible mechanism of sleeping inducement of Jiaotaiwan in homeostasis,master clock and arousal inhibition and correlation of brain areas.