灰毡毛忍冬自然变异株（湘蕾金银花）具花蕾期长、花冠不展开、可一次性采摘等优良特点，为中药材山银花的采收提供充裕时间，大大节约生产资金，具广阔应用前景。项目组系列前期研究表明，灰毡毛忍冬正常株和变异株内源激素乙烯生成量在花初开期存在明显差异，且外源乙烯与乙烯抑制剂干预不影响变异株花表型，推测内源乙烯为调控变异株特殊表型的内在原因。基于此假说，本研究以灰毡毛忍冬正常株和变异株花为材料，拟利用高通量测序技术建立其转录组和表达谱，通过生物信息学分析筛选控制乙烯生物合成和信号转导途径相关Unigene；根据相关基因序列设计引物，用RT-PCR和RACE技术克隆基因全长；在以上基础上用荧光定量PCR技术检测各基因在两品种不同花期各花部位的表达量，筛选验证差异基因；根据差异基因序列设计引物，构建正义和反义载体，转拟南芥和灰毡毛忍冬验证基因功能。通过系列研究从内源乙烯角度阐释变异株花蕾优良表型的分子机理。

The natural mutant of Lonicera macranthoides Hand.-Mazz (L. macranthoides “Xiang Lei”)has many excellent features, such as a long bud period, unexpanded corolla, one-time picking and so on, which could provide sufficient harvest time for Flos Lonicerae, and significantly save the production capital with broad application prospects. A series of preliminary studies by the project team showed that in the beginning period of flower, the production of endogenous hormone ethylene in L. macranthoides and its’ natural mutant plant had significantly difference, exogenous ethylene and ethylene inhibitor intervention could not effect the flower phenotype of L. macranthoides “Xiang Lei”, which suggested endogenous ethylene was the intrinsic reason for regulating flowering differences between the two varieties. Based on this hypothesis, in this study, L. macranthoides and its’ natural mutant’s flower were used as materials to build L. macranthoides transcriptome and expression profiling by High-throughput sequencing, and to filter the related Unigene which control ethylene biosynthesis and ethylene signal transduction pathways through bioinformatic analysis. The study is to clone the full-length gene using RT-PCR and RACE technology ,of which the primers were designed according to the ethylene-related Unigene sequences.Then to filter and verify the different genes by detecting the expressions of these genes which were different in two different varieties of different flowering and flower parts through real-time PCR technology ,of which the primers were designed according to the sequences of the full-length gene. Then according to the different genes’ sequences to design primers, the sense and antisense vectors were built and these genes were constructed and transformed into Arabidopsis thaliana and L. macranthoides to verify the genes’ function. The aim of the study is to explain the molecules mechanism from the endogenous ethylene perspective why the natural mutant of L. macranthoides showed those superior phenotype.