在药物成分分析、药物活性分析乃至环境和食品药物残留分析中，药物分析学家正面临着不同基质样品中目标分析物含量超低微（甚至低于现有分析仪器检出限）、结构性质多样，以及样品基质复杂、严重干扰分析结果可靠性的科学问题。对此，本项目拟基于分散液液微萃取(DLLME)、超分子溶剂微萃取(SMSME)技术，选择低毒性低密度长链烷酸/烷醇两亲物质为萃取剂，以具有高密度、强氢键给体能力等优越特性的六氟异丙醇(HFIP)为分散剂、自组装剂及密度调节剂，构建DLLME-SMSME双模式新型液相微萃取体系，研究新体系对不同性质药物的萃取特性和HFIP-长链烷酸/烷醇超分子溶剂对蛋白质、多糖等大分子的限进特性，并将新体系应用于不同液体、固体样品中不同性质药物的萃取。重点解决微量低密度萃取相难收集、极性药物萃取不理想及复杂生物基质等样品中药物一步直接萃取难实现的关键科学问题。培养具有创新性思维的高级药物分析专业人才。

Analytical scientists in pharmaceutical analysis are being plagued by a major scientific problem: to determine trace target analytes with diverse properties (their contents are even lower than the detection limits of advanced analytical instruments) from complex sample matrices. To solve this problem, this project will develop several novel liquid-phase microextraction systems based on the combination of dispersive liquid-liquid microextraction (DLLME) and supramolecular solvent microextraction (SMSME) technique, using hexafluoroisopropanol (HFIP, a solvent with excellent properties of high density and strong hydrogen bond donor capacity etc.) as dispersing agent, self-assembly agent as well as density-regulating agent, and long chain alkyl acids/alcohols with low toxicity and low density as extraction agents. These new systems will be used for the extraction of various drugs in a wide of polarity, proteins and polysaccharides to investigate their extraction capabilities for organic compounds with low molecular weight (most of them are drugs) and their restricted access properties against macromolecules. Moreover, these new systems will be applied to extract pharmaceutical substances or special impurities from different complex matrices such as injections, tablets, urine, plasma, animal tissue and environmental water. Importantly, several key scientific problems, including collection difficulty of low density extraction solvents at low volume, poor extraction efficiency of polar analytes, and uneasiness of one-step direct extraction of the analytes from complex samples, will be solved after the accomplishment of this project. The additional purpose of this project is to cultivate professional talents in pharmaceutical analysis with innovative thinking.