生物材料-机体细胞相互作用首先发生在材料表面，材料表面结构尤其是微结构及图案对细胞分化具有重要影响。然而，对其影响细胞分化行为的本质和深层分子机理目前仍不清楚。由于细胞在机体内各种行为实际受到不同尺度3-D微结构因素的影响和调控，因此，如何将现有二维图案研究拓展到三维微结构空间，其意义更为深远。本申请基于课题组成员在单细胞力学测量技术领域的一项突破，为破解上述难题提供了可能。研究内容主要包括：不同维度（特别是三维）微结构图案介导的力学效应和细胞力学特征研究；基因组水平的3-D微图案力学效应介导MSCs细胞向成骨细胞定向分化的作用途径及调控规律研究等。通过上述具体和关键科学问题研究，获得对微图案力学介导细胞分化机理的规律性认识，为最终利用材料表面的3-D微结构图案对细胞行为进行力学操纵和调控、进而介导特定细胞(MSCs)的定向分化奠定重要理论基础。这将是本学科领域的一个重要创新和进展。

The interaction between biomaterials and tissue cells occures firstly at the surface of the biomaterials, surface structures, especially micro-structures and patterns of materials have a significant influence on cell differentiation. However, the nature and detailed molecular mechanism for such effects still remain unknown. In fact, cell behavior is affected and regulated by factors of micro-structures with different scales in vivo. Therefore, it is more significant how to expand the current 2-D pattern studies to 3-D micro-structure levels. The present proposal provides an effective solution to the problems above, which is mainly based on a breakthrough in single cell mechnical measuring techniques achieved by one of our team members. The research contents of the present proposal include: studies on mechanical effects and cell mechanical characteristics mediated by different dimensions micro-pattern structure (especially 3-D micro-pattern ones), and studies on pathways and regulation rules of directed differentiation from MSCs to osteoblasts mediated by mechanical effects of micro-pattern on genome level. Based on above studies on specific and key scientific issues, it can be expected to obtain an insight understanding to the mechanism of cell differentiation mechanically mediated by micro-pattern. Finally, the current research will also provide important theoretical basises on mechanical manipulation and regulation of cell behaviors, as well as further directed differentiation of the specific cell (MSCs), mediated by 3-D micro-patterns on biomaterial surfaces, which will therefore be a significant innovation and progress in tissue engineering and regenerative medicine.