高羊茅（Festuca arundinacea Schreb.）是耐热性和抗冷性最强的多年生冷季型草坪草，但是其抗旱能力较弱。地下茎是储存碳水化合物和水分的重要器官，在抗旱和旱后恢复过程中发挥重要作用。因此，研究地下茎形成及其抗旱和旱后恢复的分子机制，培育根茎型高羊茅新品种具有重要理论意义和应用前景。申请人前期研究发现细胞分裂素促进高羊茅地下茎数目增加，赤霉素促进地下茎伸长，但是与之相关的基因功能和抗旱机理并不清楚。本课题拟通过①高通量iTRAQ技术和de novo 转录组测序技术从蛋白质组学和转录组学层面，探讨激素调控地下茎发育的网络机制，筛选重要调控基因和蛋白；②过量表达候选基因，获得优质根茎型高羊茅新种质；③深入研究地下茎形成在抗旱和旱后恢复作用中的调控机理。为培育抗逆和低养护高羊茅新品种提供重要基因资源和理论依据。

Tall fescue (Festuca arundinacea Schreb.) is a cool-season perennial turfgrass with the strongest heat tolerance and cold resistance, but has less drought tolerance. The rhizomes of turfgrass are the important organs which storage carbohydrates and water, and play crucial roles during drought tolerance and post-drought recovery. Therefore, it have important theoretical significance and application prospects for understanding the molecular mechanisms of drought tolerance and post-drought recovery associated with the rhizomatous formation and breeding new cultivars with rhizomes. The study of applicant has indicated that the numbers of rhizomes were increased by treatment with cytokinin and the length of them was promoted after gibberellic acid treatment in tall fescue. However, it is still unknown that genes functions and the regulation mechanisms. The objective of the present work was to ① discover the whole genome and proteome regulation network and screen the candidate genes and proteins involved in hormones signal pathway though the high throughput isobaric tags for relative and absolute quantitation (iTRAQ) and transcriptome depth sequencing technologies, ② obtain the transgenic rhizomatous tall fescues with over-expressing candidate genes, ③ illustrate the molecular mechanisms of drought tolerance and post-drought recovery associated with the rhizomatous formation. At last, this project will provide the genetic resource and theoretical basis for breeding new lower maintenance and higher resistance varieties of tall fescue.