沿海滩涂蕴育着丰富的盐生植物多样性，并形成了独特的生态系统，在土壤形成与保持、生物多样性维持、以及盐碱地生态系统的恢复与重建过程中具有重要的生态功能。内生真菌是指生活于植物组织内部，并对宿主没有引起明显伤害的一类真菌，它可促进植物生长，提高植物对盐碱等不良环境胁迫的抗性，因此，内生真菌在生态系统的演替过程中发挥重要的功能。而揭示内生真菌的群落结构与分布格局，是进一步阐明内生真菌生态功能的前提，然而，我们对滩涂生态系统中的盐生植物内生真菌知之甚少。因此，本项目以我国沿海滩涂生态系统中的禾本科常见盐生植物为对象，结合传统分离培养和非培养的高通量DNA测序技术检测内生真菌的多样性，从内生真菌的偏好性和网络结构特性上揭示内生真菌的群落结构、空间分布格局及其维持机制。本研究将为全面阐明生态系统中生物多样性的共存机理提供重要科学依据，同时收集大量内生真菌菌株，为进一步的开发利用提供丰富的真菌资源。

The coastal tidal flat ecosystems, with high halophyte diversity, play important ecological functions in soil formation, biodiversity maintenance and reestablishment. Endophytic fungi, live inside healthy plant tissues without causing overt symptoms, play important roles in improving plant growth and stress resistance, and ecosystem succession. Knowledge on the community structure and distribution pattern of endophytic fungi is fundamental to understand its ecological function in ecosystem. However, there are limited studies focused on endophytic fungi of halophytes, which widely distributed in salinized tidal flat ecosystem along the coastline in China. Our project will investigate the endophytic fungi associated with Poaceae halophytes in coastal tidal flat ecosystem in China, using traditional culture method and pyrosequencing technology. The community structure and distribution patterns of endophytic fungi will be revealed based on the analysis of host preference and plant-fungus network. This finding will improving our understand the maintenance mechanism of biodiversity in ecosystems significantly, support abundant fungal strains for further application in future.