多倍体复合体是杂交与多倍化同时作用于近缘类群而产生的重要进化现象，其中枢纽基因组是连接多倍体复合体成员的关键类群。研究证明不完全的生殖隔离无法阻止适应性基因在复合体散播，项目组近期研究表明禺毛茛复合体各类群在同域分布区存在持续且广泛的基因流，枢纽基因组与多个成员同时杂交的现象，进一步揭示了枢纽基因组在多倍体复合体进化中的重要作用，显示适应性进化可能通过枢纽基因组实现。因此，本项目拟利用群体遗传学研究方法，以自然进化的禺毛茛多倍体复合体为研究对象，利用分子标记和叶绿体单倍型对禺毛茛复合体进行基因流及遗传结构分析，结合适应性对比实验和生态位模型数据，分析不同基因型的适应性差别及其与基因流和遗传组成间的关系，验证枢纽基因组与适应性进化内在联系的假设，揭示枢纽基因组对多倍体复合体进化的影响。项目的开展将更深层次理解多倍体复合体，进一步丰富多倍体适应性进化及物种形成理论。

Polyploidization and hybridization act on related taxa leading to polyploid complex. There is a pivotal genome in polyploid complex, possessing some particular combination of adaptive characteristics, and it can tie together a cluster of related taxa through hybridization. Recent study indicated that incomplete reproductive isolation can accelerate adaptive genes spreading through polyploid complexes. According to widespread and ongoing gene flow among Ranunculus cantoniensis complex and pivotal genome hybridizing with other taxa simultaneously, the applicant inferred that complexes achieve to adaptive evolution by pivotal genome. Thus this project intends to study the gene flow and genetic structure of R. cantoniensis complex using molecular marker and chloroplast haplotype, according to the population genetics research methods, combined with the adaptive contrast experiment and ecological niche modeling to analyze the relationship of adaptability of different genotypes and gene flow, to verify the inner connection of pivotal genome and adaptive evolution, and reveal that how pivotal genome effect the evolution of polyploid complex. This project will conduct a deeper understanding of the polyploid complex, and further enrich the polyploid adaptive evolution and speciation theory.