减数分裂在有性生殖生物的生命周期中起着重要的作用。减数分裂的核心是染色体之间发生重组和交换，而重组产生的前提是减数分裂过程中的双链断链（DSB）的形成。我们前期的研究表明基因组中的遗传异质性能促进DNA断裂，进而促进突变与重组。但是杂合度对重组率的影响尚无直接的证据。为了进一步直接检验异质性对减数分裂重组的影响，本研究拟利用研究蛋白-DNA相互作用的ChIP-seq技术来直接检测不同杂合度个体DSB的数量与突变的关系。同时，利用CHIP-seq，还能够获得植物减数分裂DSB首张精细图谱。本研究拟揭示减数分裂重组及影响其产生与分布的分子机制，并进一步深入理解生物适应与演化的内在分子机制。

Meiosis plays a central role in the life cycles of all sexually reproductive organisms. The core of meiosis is the recombination and exchange between chromosomes and the precondition of generation of meiotic recombination is the formation of double-strand breaks (DSBs). Our previous study shows that the heterozygosity of the genome promotes the break of DNA and then mutation and recombination. However, there is no direct evidence for the effect of heterozygosity to recombination. To test the effect of heterozygosity to recombination, this study will use ChIP-seq, the popular technology to analyze protein interactions with DNA, to analyze the relationship between the number of DSB and mutation in individuals with different heterozygosity. Using ChIP-seq, we can acquire a fine map of all the meiotic recombination hot spots. This project will not only reveal the law and impact on the generation and distribution of meiotic recombination but also help to understand the inner base of adaption and evolution.