Bacillomycin D (BD)是一种由芽孢杆菌产生的抗菌脂肽，高效抑制黄曲霉和赭曲霉菌，并减少真菌毒素的产生，因此BD在减少粮食真菌毒素污染和粮食损失等方面具有潜在的应用前景。国内外对BD合成酶基因已有较多研究，但尚无阐明BD合成的营养代谢调控和基因水平的调控机理，影响了在基因水平改良BD高产菌株。本研究旨在利用BD生产菌株B. Subtilis，通过在不同营养水平下BD合成的差异，结合RT-PCR技术、转录组学和代谢组学的手段，探索影响BD合成的关键酶和调控因子，通过敲除或过表达关键酶和调控蛋白的基因以及sRNA，分析与合成代谢过程的关系，确定关键酶及调控蛋白对BD合成基因的转录和翻译的调控作用，阐明BD生物合成的代谢调控机理，为从分子水平上改良BD高产菌株以及高效生产BD提供科学依据。

Bacillomycin D (BD) is an antifungal lipopeptide produced by Bacillus sp. It inhibits efficiently Aspergillus flavus and A. ochraceus and reduces the production of mycotoxins. So, BD has a promising prospect to be applied to reduce the pollution of mycotoxins and loss in grain. Many studies on BD synthase gene have been widely reported at abroad, however, there is few works on the nutrition metabolic and gene regulation mechanism of BD biosynthesis, which has affected the breeding of BD high-yield strains at the genetic level. In order to elaborate the metabolic regulation mechanism of BD biosynthesis, we will explore the key enzymes and regulation factors on BD synthesis by combined with RT-PCR, transcriptomics and metabolomics techniques under different nutrient levels. The effect of knockout and overexpression of key enzymes, regulatory signal protein genes and small RNA in B. subtilis on BD synthesis will be studied and the relationship between these regulation factors and transcription, expression of BD synthase gene or synthesis metabolic pathway will be investigated. Our results provide a scientific basis for improving the production of BD in B. subtilis at molecular level.