中文摘要
干旱严重制约花生的产量与品质。目前对花生在旱后恢复生长的研究甚少,调节机制不清楚。课题组前期从花生去乙酰化酶的酵母库中筛选克隆出一个GLK类转录因子基因。花生在干旱下该基因表达快速下降,复水恢复生长后表达逐渐上调,其影响叶绿素合成关键酶AhPORA基因启动子转录活性,命名为花生旱后生长恢复因子1(AhPGRF1)。在此基础上,本项目拟研究花生旱后恢复生长过程中AhPGRF1基因和蛋白表达的作用,明确AhPGRF1与组蛋白去乙酰化的相互关系, 探讨AhPGRF1与AhHDA1互作位点及其复合体在干旱及旱后恢复生长过程中的动态变化,以及对靶基因(AhPORA)的转录调节,阐明AhPGRF1与组蛋白乙酰化修饰的关系及其在花生旱后恢复生长中的功能。研究结果为揭示AhPGRF1调节花生旱后恢复生长的分子机制提供依据,促进对作物旱后恢复生长机制的深入了解。
英文摘要
The crop yield and quality of peanut could be significantly restrained by drought. However, the study on peanuts growth recovery after drought is rare and the regulating mechanism is not clear at present. Recently, a GLK-like transcription factor has been screened from the yeast cDNA library by AhHDA1. Peanut leaves become wilting together with a rapid decline of the gene expression under drought treatment during 72 to 96 h. The expression of GLK-like gene was gradually increasing after rehydration, and the expression of which promoted the transcription activity of AhPORA, an key gene of chlorophyll synthesis . The GLK-like gene was renamed as AhPGRF1 (Arachis hypogaea Post-Drought Growth Recover Factor 1). On the basis of this, this project intends to study the function of AhPGRF1 gene expression and protein expression in the process of growth recovery after drought. It will be understand the relationship between AhPGRF1 with histone acetylation .The transcription regulation mechanism of histone acetylation modification of AhPGRF1 and the effect of growth recovery after drought mediated by AhHDA1 will be studied. The results will provide basis to uncover the molecular mechanism of growth recovery after drought by AhPGRF1, and then an understanding of the mechanism growth recovery after drought will be promoted.
