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花生旱后恢复生长因子AhPGRF1的功能研究

花生旱后恢复生长因子AhPGRF1的功能研究
  • 导航:首页 > 科学基金
  • 批准号:31671600
  • 批准年度: 2016年
  • 学科分类:作物逆境生理(C130204) |
  • 项目负责人:李玲
  • 负责人职称:教授
  • 依托单位:华南师范大学
  • 资助金额:62万元
  • 项目类别:面上项目
  • 研究期限:2017年01月01日 至 2020年12月31日
  • 中文关键词: 花生;恢复;生长;因子;AhPGRF1
  • 英文关键词:drought stress;oil crops;tolerance;response to drought;molecular response

项目摘要

中文摘要

干旱严重制约花生的产量与品质。目前对花生在旱后恢复生长的研究甚少,调节机制不清楚。课题组前期从花生去乙酰化酶的酵母库中筛选克隆出一个GLK类转录因子基因。花生在干旱下该基因表达快速下降,复水恢复生长后表达逐渐上调,其影响叶绿素合成关键酶AhPORA基因启动子转录活性,命名为花生旱后生长恢复因子1(AhPGRF1)。在此基础上,本项目拟研究花生旱后恢复生长过程中AhPGRF1基因和蛋白表达的作用,明确AhPGRF1与组蛋白去乙酰化的相互关系, 探讨AhPGRF1与AhHDA1互作位点及其复合体在干旱及旱后恢复生长过程中的动态变化,以及对靶基因(AhPORA)的转录调节,阐明AhPGRF1与组蛋白乙酰化修饰的关系及其在花生旱后恢复生长中的功能。研究结果为揭示AhPGRF1调节花生旱后恢复生长的分子机制提供依据,促进对作物旱后恢复生长机制的深入了解。

英文摘要

The crop yield and quality of peanut could be significantly restrained by drought. However, the study on peanuts growth recovery after drought is rare and the regulating mechanism is not clear at present. Recently, a GLK-like transcription factor has been screened from the yeast cDNA library by AhHDA1. Peanut leaves become wilting together with a rapid decline of the gene expression under drought treatment during 72 to 96 h. The expression of GLK-like gene was gradually increasing after rehydration, and the expression of which promoted the transcription activity of AhPORA, an key gene of chlorophyll synthesis . The GLK-like gene was renamed as AhPGRF1 (Arachis hypogaea Post-Drought Growth Recover Factor 1). On the basis of this, this project intends to study the function of AhPGRF1 gene expression and protein expression in the process of growth recovery after drought. It will be understand the relationship between AhPGRF1 with histone acetylation .The transcription regulation mechanism of histone acetylation modification of AhPGRF1 and the effect of growth recovery after drought mediated by AhHDA1 will be studied. The results will provide basis to uncover the molecular mechanism of growth recovery after drought by AhPGRF1, and then an understanding of the mechanism growth recovery after drought will be promoted.

评估说明

    国家自然科学基金项目“花生旱后恢复生长因子AhPGRF1的功能研究”发布于爱科学iikx,并永久归类于相关科学基金导航中,仅供广大科研工作者查询、学习、选题参考。国科金是根据国家发展科学技术的方针、政策和规划,以及科学技术发展方向,面向全国资助基础研究和应用研究,发挥着促进我国基础研究源头创新的作用。国科金的真正价值在于它能否为科学进步和社会发展带来积极的影响。

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